Photochemical Mechanisms of Fluorophores Employed in Single-Molecule Localization Microscopy

Kai Kikuchi, Liam D. Adair, Jiarun Lin, Elizabeth J. New, Amandeep Kaur

Research output: Contribution to journalReview ArticleResearchpeer-review

24 Citations (Scopus)


Decoding cellular processes requires visualization of the spatial distribution and dynamic interactions of biomolecules. It is therefore not surprising that innovations in imaging technologies have facilitated advances in biomedical research. The advent of super-resolution imaging technologies has empowered biomedical researchers with the ability to answer long-standing questions about cellular processes at an entirely new level. Fluorescent probes greatly enhance the specificity and resolution of super-resolution imaging experiments. Here, we introduce key super-resolution imaging technologies, with a brief discussion on single-molecule localization microscopy (SMLM). We evaluate the chemistry and photochemical mechanisms of fluorescent probes employed in SMLM. This Review provides guidance on the identification and adoption of fluorescent probes in single molecule localization microscopy to inspire the design of next-generation fluorescent probes amenable to single-molecule imaging.

Original languageEnglish
Article numbere202204745
Number of pages25
JournalAngewandte Chemie - International Edition
Issue number1
Publication statusPublished - 2 Jan 2023


  • Fluorescence
  • Photochemistry
  • Photoswitching
  • Sensors
  • Super-Resolution

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