Phosphorylation and activation of tryptophan hydroxylase by exogenous protein kinase A

Patricia A. Johansen, Ian Jennings, Richard G H Cotton, Donald M. Kuhn

Research output: Contribution to journalArticleResearchpeer-review

45 Citations (Scopus)

Abstract

The catalytic subunit of protein kinase A increases brain tryptophan hydroxylase activity. The activation is manifested as an increase in V(max) without alterations in the K(m) for either tetrahydrobiopterin or tryptophan. The activation of tryptophan hydroxylase by protein kinase A is dependent on ATP and an intact kinase and is inhibited specifically by protein kinase A inhibitors. Protein kinase A also catalyzes the phosphorylation of tryptophan hydroxylase. The extent to which tryptophan hydroxylase is phosphorylated by protein kinase A is dependent on the amount of kinase used and is closely related to the degree to which the hydroxylase is activated. These results suggest that a direct relationship exists between phosphorylation and activation of tryptophan hydroxylase by protein kinase A.

Original languageEnglish
Pages (from-to)817-823
Number of pages7
JournalJournal of Neurochemistry
Volume66
Issue number2
Publication statusPublished - 1 Feb 1996
Externally publishedYes

Keywords

  • Activation
  • Phosphorylation
  • Protein kinase A
  • Tryptophan hydroxylase

Cite this

Johansen, Patricia A. ; Jennings, Ian ; Cotton, Richard G H ; Kuhn, Donald M. / Phosphorylation and activation of tryptophan hydroxylase by exogenous protein kinase A. In: Journal of Neurochemistry. 1996 ; Vol. 66, No. 2. pp. 817-823.
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Phosphorylation and activation of tryptophan hydroxylase by exogenous protein kinase A. / Johansen, Patricia A.; Jennings, Ian; Cotton, Richard G H; Kuhn, Donald M.

In: Journal of Neurochemistry, Vol. 66, No. 2, 01.02.1996, p. 817-823.

Research output: Contribution to journalArticleResearchpeer-review

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