Phenotypic characterization of blood monocytes from HIV-infected individuals

Philip Ellery, Suzanne Mary Crowe

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Other

9 Citations (Scopus)

Abstract

Monocytes play an important, yet only partly understood, role in HIV-1 pathogenesis. Two main subsets of peripheral blood monocytes have been described; the major subset of monocytes are phenotypically characterized as being CD14hi/CD16a??, and a minor subset (5a??15 of total monocytes in healthy individuals), which are CD14lo/CD16hi, have been reported to be expanded in HIV-infected individuals. These CD14lo/CD16hi monocytes differ from the majority of monocytes in a number of ways, including the molecules expressed on their surface and how they function. Here we describe a flow-cytometric assay to identify and compare the expression of a representative surface molecule (CCR5) on CD14hi/CD16a?? and CD14lo/CD16hi monocytes in small volumes of whole blood, and methods to isolate monocyte subsets by both fluorescence-activated cell sorting (FACS) and magnetic bead sorting.
Original languageEnglish
Title of host publicationMethods in Molecular Biology - Human Retrovirus Protocols: Virology and Molecular Biology
EditorsTuofu Zhu
Place of PublicationUSA
PublisherHumana Press
Pages343 - 353
Number of pages11
Edition304
ISBN (Print)9781588294951
Publication statusPublished - 2005

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