Phasing possibilities using different wavelengths with a xenon derivative

Santosh Panjikar, Paul A. Tucker

Research output: Contribution to journalArticleResearchpeer-review


Xenon derivatives are generally expected to be isomorphous with the native; however, the K- and L-absorption edges are not easily accessible on most synchrotron beamlines, which might limit their usefulness in phase determination. Various phasing procedures for xenon-derivatized porcine pancreatic elastase have been investigated using data sets measured at three generally accessible wavelengths. The importance of highly redundant data in measuring precise anomalous differences is highlighted and it is shown that, after such measurements, a single isomorphous replacement anomalous scattering (SIRAS) procedure yields a better phase set than those generated by single anomalous scattering (SAS) or multiwavelength anomalous diffraction (MAD) procedures.

Original languageEnglish
Pages (from-to)261-266
Number of pages6
JournalJournal of Applied Crystallography
Issue number2
Publication statusPublished - 2002
Externally publishedYes

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