Phage display - A powerful genetic probe of biomolecular interactions

Claire Mitchell, Vincent R. Harley

Research output: Contribution to journalReview ArticleResearchpeer-review

2 Citations (Scopus)

Abstract

Phage display is about expressing a library of proteins or peptides on the surface of phages and selecting that which binds ligand with highest affinity. It exploits the filamentous bacteriophages, which can incorporate recombinant protein into the coat and package the DNA encoding it. Two features make phage display unique: affinity selection and the physical link between the recombinant protein and the DNA encoding it. From a huge library of protein variants, the variant which interacts with highest affinity is selected, amplified in bacteria and its DNA sequenced. Here we provide an overview of some of the more interesting and imaginative applications of phage display to biology and medicine.

Original languageEnglish
Pages (from-to)218-222
Number of pages5
JournalAustralasian Biotechnology
Volume7
Issue number4
Publication statusPublished - 1 Jan 1997
Externally publishedYes

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