Abstract
Phage display is about expressing a library of proteins or peptides on the surface of phages and selecting that which binds ligand with highest affinity. It exploits the filamentous bacteriophages, which can incorporate recombinant protein into the coat and package the DNA encoding it. Two features make phage display unique: affinity selection and the physical link between the recombinant protein and the DNA encoding it. From a huge library of protein variants, the variant which interacts with highest affinity is selected, amplified in bacteria and its DNA sequenced. Here we provide an overview of some of the more interesting and imaginative applications of phage display to biology and medicine.
Original language | English |
---|---|
Pages (from-to) | 218-222 |
Number of pages | 5 |
Journal | Australasian Biotechnology |
Volume | 7 |
Issue number | 4 |
Publication status | Published - 1 Jan 1997 |
Externally published | Yes |