PH-sensitive name-crystals of carbonate apatite regulate delivery and release kinetics of DNA for efficient expression in mammalian cells

E. H. Chowdhury, Toshihiro Akaike

Research output: Chapter in Book/Report/Conference proceedingConference PaperResearchpeer-review

Abstract

Due to some major limitations of viral-mediated delivery, non-viral synthetic systems have become increasingly desirable. However, synthetic systems are notably inefficient compared to the viral ones in gene delivery and expression. Here we report on the development of the simplest, but highly efficient gene delivery device based on generated nano-apatite crystals having high affinity to DNA but fast dissolution kinetics in acidic vesicles, following efficient endocytosis, for effective release of DNA and thus result in 5 to 100-fold higher transgene expression than the existing ones. Fluoride (F -) or strontium (Sr2+) which is known to decrease the solubility of carbonate apatite, dramatically reduced the transfection efficiency, suggesting that DNA release through particle dissolution, is a crucial factor in gene delivery pathway. Additionally, flexibility in modulating crystal dissolution kinetics enabled to control intracellular DNA release and an intermediate rate of DNA release enhanced survival of DNA and subsequent expression.

Original languageEnglish
Title of host publication2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 Technical Proceedings
EditorsM. Laudon, B. Romanowicz
Pages254-257
Number of pages4
Publication statusPublished - 2005
Externally publishedYes
EventNanotechnology Conference and Trade Show (Nanotech) 2005 - Anaheim, California, USA, Anaheim, United States of America
Duration: 8 May 200512 May 2005

Conference

ConferenceNanotechnology Conference and Trade Show (Nanotech) 2005
Abbreviated titleNSTI Nanotech 2005
CountryUnited States of America
CityAnaheim
Period8/05/0512/05/05

Keywords

  • Acid solubility
  • Calcium phosphate
  • Carbonate apatite
  • DNA release
  • Endosomes
  • Hydroxyapatite
  • Transfection

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