pH-Responsive Transferrin-pHlexi particles capable of targeting cells in vitro

Adelene S.M. Wong; Ewa Czuba; Moore Z. Chen; Daniel Yuen; Kristofer I. Cupic; Shenglin Yang; Rebecca Y. Hodgetts; Laura I. Selby; Angus P.R. Johnston; Georgina Kate Such

doi:10.1021/acsmacrolett.7b00044

pH-Responsive Transferrin-pHlexi particles capable of targeting cells in vitro

Adelene S.M. Wong, Ewa Czuba, Moore Z. Chen, Daniel Yuen, Kristofer I. Cupic, Shenglin Yang, Rebecca Y. Hodgetts, Laura I. Selby, Angus P.R. Johnston, Georgina Kate Such

Drug Delivery Disposition & Dynamics

Research output: Contribution to journal › Article › Research › peer-review

14 Citations (Scopus)

Abstract

Targeting nanoparticles to specific cellular receptors has the potential to deliver therapeutic compounds to target sites while minimizing side effects. To this end, we have conjugated a targeting protein, holo-Transferrin (holo-Tf), to pH-responsive polymers, poly(2-(diethylamino)ethyl methacrylate) (PDEAEMA) and poly(2-(diethylamino)ethyl methacrylate)-ran-poly(2-(diisopropylamino)ethyl methacrylate (PDEAEMA-r-PDPAEMA). These protein-polymer hybrid materials were observed to self-Assemble when the pH is increased above the pK_a of the polymer. We demonstrate that their response to pH could be tuned depending on the polymer constituent attached to holo-Tf. Importantly, the targeting behavior of these nanoparticles could be maximized by tuning the density of holo-Tf on the nanoparticle surface by the introduction of a (PDEAEMA-r-PDPAEMA)-b-poly(ethylene glycol) (PEG) copolymer.

Original language	English
Pages (from-to)	315-320
Number of pages	6
Journal	ACS Macro Letters
Volume	6
Issue number	3
DOIs	https://doi.org/10.1021/acsmacrolett.7b00044
Publication status	Published - 2017

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10.1021/acsmacrolett.7b00044

106872996-oaAccepted author manuscript, 24.6 MBLicence: Unspecified

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@article{707b491bb6b2425681bf136b0f1eae49,

title = "pH-Responsive Transferrin-pHlexi particles capable of targeting cells in vitro",

abstract = "Targeting nanoparticles to specific cellular receptors has the potential to deliver therapeutic compounds to target sites while minimizing side effects. To this end, we have conjugated a targeting protein, holo-Transferrin (holo-Tf), to pH-responsive polymers, poly(2-(diethylamino)ethyl methacrylate) (PDEAEMA) and poly(2-(diethylamino)ethyl methacrylate)-ran-poly(2-(diisopropylamino)ethyl methacrylate (PDEAEMA-r-PDPAEMA). These protein-polymer hybrid materials were observed to self-Assemble when the pH is increased above the pKa of the polymer. We demonstrate that their response to pH could be tuned depending on the polymer constituent attached to holo-Tf. Importantly, the targeting behavior of these nanoparticles could be maximized by tuning the density of holo-Tf on the nanoparticle surface by the introduction of a (PDEAEMA-r-PDPAEMA)-b-poly(ethylene glycol) (PEG) copolymer.",

author = "Wong, {Adelene S.M.} and Ewa Czuba and Chen, {Moore Z.} and Daniel Yuen and Cupic, {Kristofer I.} and Shenglin Yang and Hodgetts, {Rebecca Y.} and Selby, {Laura I.} and Johnston, {Angus P.R.} and Such, {Georgina Kate}",

year = "2017",

doi = "10.1021/acsmacrolett.7b00044",

language = "English",

volume = "6",

pages = "315--320",

journal = "ACS Macro Letters",

issn = "2161-1653",

publisher = "American Chemical Society",

number = "3",

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T1 - pH-Responsive Transferrin-pHlexi particles capable of targeting cells in vitro

AU - Wong, Adelene S.M.

AU - Czuba, Ewa

AU - Chen, Moore Z.

AU - Yuen, Daniel

AU - Cupic, Kristofer I.

AU - Yang, Shenglin

AU - Hodgetts, Rebecca Y.

AU - Selby, Laura I.

AU - Johnston, Angus P.R.

AU - Such, Georgina Kate

PY - 2017

Y1 - 2017

N2 - Targeting nanoparticles to specific cellular receptors has the potential to deliver therapeutic compounds to target sites while minimizing side effects. To this end, we have conjugated a targeting protein, holo-Transferrin (holo-Tf), to pH-responsive polymers, poly(2-(diethylamino)ethyl methacrylate) (PDEAEMA) and poly(2-(diethylamino)ethyl methacrylate)-ran-poly(2-(diisopropylamino)ethyl methacrylate (PDEAEMA-r-PDPAEMA). These protein-polymer hybrid materials were observed to self-Assemble when the pH is increased above the pKa of the polymer. We demonstrate that their response to pH could be tuned depending on the polymer constituent attached to holo-Tf. Importantly, the targeting behavior of these nanoparticles could be maximized by tuning the density of holo-Tf on the nanoparticle surface by the introduction of a (PDEAEMA-r-PDPAEMA)-b-poly(ethylene glycol) (PEG) copolymer.

AB - Targeting nanoparticles to specific cellular receptors has the potential to deliver therapeutic compounds to target sites while minimizing side effects. To this end, we have conjugated a targeting protein, holo-Transferrin (holo-Tf), to pH-responsive polymers, poly(2-(diethylamino)ethyl methacrylate) (PDEAEMA) and poly(2-(diethylamino)ethyl methacrylate)-ran-poly(2-(diisopropylamino)ethyl methacrylate (PDEAEMA-r-PDPAEMA). These protein-polymer hybrid materials were observed to self-Assemble when the pH is increased above the pKa of the polymer. We demonstrate that their response to pH could be tuned depending on the polymer constituent attached to holo-Tf. Importantly, the targeting behavior of these nanoparticles could be maximized by tuning the density of holo-Tf on the nanoparticle surface by the introduction of a (PDEAEMA-r-PDPAEMA)-b-poly(ethylene glycol) (PEG) copolymer.

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DO - 10.1021/acsmacrolett.7b00044

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SN - 2161-1653

VL - 6

SP - 315

EP - 320

JO - ACS Macro Letters

JF - ACS Macro Letters

IS - 3

ER -

pH-Responsive Transferrin-pHlexi particles capable of targeting cells in vitro

Abstract

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ARC Centre of Excellence in Convergent Bio-Nano Science and Technology

Bridging the Interface between Nanoengineered Materials and Biological Systems

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pH-Responsive Transferrin-pHlexi particles capable of targeting cells in vitro

Abstract

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Projects

ARC Centre of Excellence in Convergent Bio-Nano Science and Technology

Bridging the Interface between Nanoengineered Materials and Biological Systems

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