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Petrobactin biosynthesis: AsbB catalyzes condensation of spermidine with N8-citryl-spermidine and its N1-(3,4-dihydroxybenzoyl) derivative

  • Daniel Oves-Costales
  • , Nadia Kadi
  • , Mark J. Fogg
  • , Lijiang Song
  • , Keith S. Wilson
  • , Gregory L. Challis

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The AsbB enzyme, which is involved in the biosynthesis of the virulence-conferring siderophore petrobactin in Bacillus anthracis, is shown to catalyze efficient ATP-dependent condensation of spermidine, but not N 1-(3,4-dihydroxbenzoyl)-spermidine, with N8-citryl- spermidine or N1-(3,4-dihydroxbenzoyl)-N8-citryl- spermidine, suggesting that N1-(3,4-dihydroxbenzoyl)-spermidine is very unlikely to be a significant intermediate in petrobactin biosynthesis, contrary to previous suggestions.

Original languageEnglish
Pages (from-to)4034-4036
Number of pages3
JournalChemical Communications
Issue number34
DOIs
Publication statusPublished - 8 Sept 2008
Externally publishedYes

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