Perspectives on precision cut lung slices—powerful tools for investigation of mechanisms and therapeutic targets in lung diseases

Maggie Lam, Emma Lamanna, Louise Organ, Chantal Donovan, Jane E. Bourke

Research output: Contribution to journalReview ArticleResearchpeer-review

9 Citations (Scopus)

Abstract

Precision cut lung slices (PCLS) have emerged as powerful experimental tools for respiratory research. Pioneering studies using mouse PCLS to visualize intrapulmonary airway contractility have been extended to pulmonary arteries and for assessment of novel bronchodilators and vasodilators as therapeutics. Additional disease-relevant outcomes, including inflammatory, fibrotic, and regenerative responses, are now routinely measured in PCLS from multiple species, including humans. This review provides an overview of established and innovative uses of PCLS as an intermediary between cellular and organ-based studies and focuses on opportunities to increase their application to investigate mechanisms and therapeutic targets to oppose excessive airway contraction and fibrosis in lung diseases.

Original languageEnglish
Article number1162889
Number of pages14
JournalFrontiers in Pharmacology
Volume14
DOIs
Publication statusPublished - 2023

Keywords

  • airway
  • bronchodilator
  • fibrosis
  • intrapulmonary artery
  • PCLS
  • standardisation
  • vasodilator

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