Perivascular macrophages mediate neutrophil recruitment during bacterial skin infection

Arby Abtin, Rohit Jain, Andrew J Mitchell, Ben Roediger, Anthony J Brzoska, Shweta Tikoo, Qiang Cheng, Lai Guan Ng, Lois L Cavanagh, Ulrich H von Andrian, Michael John Hickey, Neville Firth, Wolfgang Weninger

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Transendothelial migration of neutrophils in postcapillary venules is a key event in the inflammatory response against pathogens and tissue damage. The precise regulation of this process is incompletely understood. We report that perivascular macrophages are critical for neutrophil migration into skin infected with the pathogen Staphylococcus aureus. Using multiphoton intravital microscopy we showed that neutrophils extravasate from inflamed dermal venules in close proximity to perivascular macrophages, which are a major source of neutrophil chemoattractants. The virulence factor alpha-hemolysin produced by S. aureus lyses perivascular macrophages, which leads to decreased neutrophil transmigration. Our data illustrate a previously unrecognized role for perivascular macrophages in neutrophil recruitment to inflamed skin and indicate that S. aureus uses hemolysin-dependent killing of these cells as an immune evasion strategy.
Original languageEnglish
Pages (from-to)45 - 53
Number of pages9
JournalNature Immunology
Volume15
Issue number1
DOIs
Publication statusPublished - 2014

Cite this

Abtin, A., Jain, R., Mitchell, A. J., Roediger, B., Brzoska, A. J., Tikoo, S., ... Weninger, W. (2014). Perivascular macrophages mediate neutrophil recruitment during bacterial skin infection. Nature Immunology, 15(1), 45 - 53. https://doi.org/10.1038/ni.2769
Abtin, Arby ; Jain, Rohit ; Mitchell, Andrew J ; Roediger, Ben ; Brzoska, Anthony J ; Tikoo, Shweta ; Cheng, Qiang ; Ng, Lai Guan ; Cavanagh, Lois L ; von Andrian, Ulrich H ; Hickey, Michael John ; Firth, Neville ; Weninger, Wolfgang. / Perivascular macrophages mediate neutrophil recruitment during bacterial skin infection. In: Nature Immunology. 2014 ; Vol. 15, No. 1. pp. 45 - 53.
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abstract = "Transendothelial migration of neutrophils in postcapillary venules is a key event in the inflammatory response against pathogens and tissue damage. The precise regulation of this process is incompletely understood. We report that perivascular macrophages are critical for neutrophil migration into skin infected with the pathogen Staphylococcus aureus. Using multiphoton intravital microscopy we showed that neutrophils extravasate from inflamed dermal venules in close proximity to perivascular macrophages, which are a major source of neutrophil chemoattractants. The virulence factor alpha-hemolysin produced by S. aureus lyses perivascular macrophages, which leads to decreased neutrophil transmigration. Our data illustrate a previously unrecognized role for perivascular macrophages in neutrophil recruitment to inflamed skin and indicate that S. aureus uses hemolysin-dependent killing of these cells as an immune evasion strategy.",
author = "Arby Abtin and Rohit Jain and Mitchell, {Andrew J} and Ben Roediger and Brzoska, {Anthony J} and Shweta Tikoo and Qiang Cheng and Ng, {Lai Guan} and Cavanagh, {Lois L} and {von Andrian}, {Ulrich H} and Hickey, {Michael John} and Neville Firth and Wolfgang Weninger",
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Abtin, A, Jain, R, Mitchell, AJ, Roediger, B, Brzoska, AJ, Tikoo, S, Cheng, Q, Ng, LG, Cavanagh, LL, von Andrian, UH, Hickey, MJ, Firth, N & Weninger, W 2014, 'Perivascular macrophages mediate neutrophil recruitment during bacterial skin infection', Nature Immunology, vol. 15, no. 1, pp. 45 - 53. https://doi.org/10.1038/ni.2769

Perivascular macrophages mediate neutrophil recruitment during bacterial skin infection. / Abtin, Arby; Jain, Rohit; Mitchell, Andrew J; Roediger, Ben; Brzoska, Anthony J; Tikoo, Shweta; Cheng, Qiang; Ng, Lai Guan; Cavanagh, Lois L; von Andrian, Ulrich H; Hickey, Michael John; Firth, Neville; Weninger, Wolfgang.

In: Nature Immunology, Vol. 15, No. 1, 2014, p. 45 - 53.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Tikoo, Shweta

AU - Cheng, Qiang

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Abtin A, Jain R, Mitchell AJ, Roediger B, Brzoska AJ, Tikoo S et al. Perivascular macrophages mediate neutrophil recruitment during bacterial skin infection. Nature Immunology. 2014;15(1):45 - 53. https://doi.org/10.1038/ni.2769