Peptide-Pegylated Lipid Conjugation Via Copper-Catalyzed Alkyne-Azide 1,3-Dipolar Cycloaddition

Waleed M. Hussein; Istvan Toth

doi:10.1007/978-1-0716-1617-8_6

Peptide-Pegylated Lipid Conjugation Via Copper-Catalyzed Alkyne-Azide 1,3-Dipolar Cycloaddition

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

Abstract

Targeted drug delivery is an important strategy in the treatment of many diseases. However, cancer cells are very difficult to target, making this a substantial obstacle in chemotherapy treatment. Bombesin fragment (BBN(6–14)) has been found to target gastrin-releasing peptide receptor (GRPR), which is overexpressed in many cancer cells. In this chapter, BBN peptide was used as a targeting moiety on the surface of polymeric-based nanoparticles to deliver its payload into prostate cancer PC-3 cell lines. Copper-catalyzed alkyne-azide 1,3-dipolar cycloaddition (CuAAC) click reaction was utilized to link the BBN peptide with an alkyne derivative of Pegylated lipid.

Original language	English
Title of host publication	Peptide Conjugation
Subtitle of host publication	Methods and Protocols
Editors	Waleed M Hussein, Rachel J Stephenson, Istvan Toth
Place of Publication	New York, USA
Publisher	Humana Press
Chapter	6
Pages	57-63
Number of pages	7
Volume	2355
ISBN (Electronic)	978-1-0716-1617-8
ISBN (Print)	978-1-0716-1616-1
DOIs	https://doi.org/10.1007/978-1-0716-1617-8_6
Publication status	Published - 2021
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	2355
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Alkyne-azide cycloaddition
Bombesin
Click chemistry
Polyethylene glycol linker
Targeting peptide

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/978-1-0716-1617-8_6

Cite this

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title = "Peptide-Pegylated Lipid Conjugation Via Copper-Catalyzed Alkyne-Azide 1,3-Dipolar Cycloaddition",

abstract = "Targeted drug delivery is an important strategy in the treatment of many diseases. However, cancer cells are very difficult to target, making this a substantial obstacle in chemotherapy treatment. Bombesin fragment (BBN(6–14)) has been found to target gastrin-releasing peptide receptor (GRPR), which is overexpressed in many cancer cells. In this chapter, BBN peptide was used as a targeting moiety on the surface of polymeric-based nanoparticles to deliver its payload into prostate cancer PC-3 cell lines. Copper-catalyzed alkyne-azide 1,3-dipolar cycloaddition (CuAAC) click reaction was utilized to link the BBN peptide with an alkyne derivative of Pegylated lipid.",

keywords = "Alkyne-azide cycloaddition, Bombesin, Click chemistry, Polyethylene glycol linker, Targeting peptide",

author = "Hussein, \{Waleed M.\} and Istvan Toth",

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series = "Methods in Molecular Biology",

publisher = "Humana Press",

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booktitle = "Peptide Conjugation",

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Peptide-Pegylated Lipid Conjugation Via Copper-Catalyzed Alkyne-Azide 1,3-Dipolar Cycloaddition. / Hussein, Waleed M.; Toth, Istvan.
Peptide Conjugation: Methods and Protocols. ed. / Waleed M Hussein; Rachel J Stephenson; Istvan Toth. Vol. 2355 New York, USA: Humana Press, 2021. p. 57-63 (Methods in Molecular Biology; Vol. 2355).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

TY - CHAP

T1 - Peptide-Pegylated Lipid Conjugation Via Copper-Catalyzed Alkyne-Azide 1,3-Dipolar Cycloaddition

AU - Hussein, Waleed M.

AU - Toth, Istvan

PY - 2021

Y1 - 2021

N2 - Targeted drug delivery is an important strategy in the treatment of many diseases. However, cancer cells are very difficult to target, making this a substantial obstacle in chemotherapy treatment. Bombesin fragment (BBN(6–14)) has been found to target gastrin-releasing peptide receptor (GRPR), which is overexpressed in many cancer cells. In this chapter, BBN peptide was used as a targeting moiety on the surface of polymeric-based nanoparticles to deliver its payload into prostate cancer PC-3 cell lines. Copper-catalyzed alkyne-azide 1,3-dipolar cycloaddition (CuAAC) click reaction was utilized to link the BBN peptide with an alkyne derivative of Pegylated lipid.

AB - Targeted drug delivery is an important strategy in the treatment of many diseases. However, cancer cells are very difficult to target, making this a substantial obstacle in chemotherapy treatment. Bombesin fragment (BBN(6–14)) has been found to target gastrin-releasing peptide receptor (GRPR), which is overexpressed in many cancer cells. In this chapter, BBN peptide was used as a targeting moiety on the surface of polymeric-based nanoparticles to deliver its payload into prostate cancer PC-3 cell lines. Copper-catalyzed alkyne-azide 1,3-dipolar cycloaddition (CuAAC) click reaction was utilized to link the BBN peptide with an alkyne derivative of Pegylated lipid.

KW - Alkyne-azide cycloaddition

KW - Bombesin

KW - Click chemistry

KW - Polyethylene glycol linker

KW - Targeting peptide

UR - https://www.scopus.com/pages/publications/85113786547

U2 - 10.1007/978-1-0716-1617-8_6

DO - 10.1007/978-1-0716-1617-8_6

M3 - Chapter (Book)

C2 - 34386950

AN - SCOPUS:85113786547

SN - 978-1-0716-1616-1

VL - 2355

T3 - Methods in Molecular Biology

SP - 57

EP - 63

BT - Peptide Conjugation

A2 - Hussein, Waleed M

A2 - Stephenson, Rachel J

A2 - Toth, Istvan

PB - Humana Press

CY - New York, USA

ER -

Peptide-Pegylated Lipid Conjugation Via Copper-Catalyzed Alkyne-Azide 1,3-Dipolar Cycloaddition

Abstract

Publication series

Keywords

UN SDGs

Access to Document

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Cite this