Peptide-functionalized, low-biofouling click multilayers for promoting cell adhesion and growth

Cameron R Kinnane, Kim Wark, Georgina Kate Such, Angus Johnston, Frank Caruso

Research output: Contribution to journalArticleResearchpeer-review

48 Citations (Scopus)

Abstract

The preparation of low-fouling poly(ethylene glycol) (PEG) acrylate multilayers through the click-layer-by-layer (LbL) technique was reported. Radical polymerization was used to synthesize PEG-acrylate polymers with alkyne or azide functionalities. Nuclear magnetic resonance (NMR) spectroscopy show that PEG-Az contain approximately 15 azide groups and PEG-Alk 40 alkyne groups. It is also found that peptides can be coupled to the PEGylated surface by the attachment of GRGDSP-propargyl Gly, through analysis by ellipsometry. The potential of the PEG films to provide a low-biofouling surface assessed by comparing cell adhesion on untreated glass and PEG multilayers show that these cell surfaces have an elongated or flattened profile, exhibiting epithelial morphology. A steady growth of cells is observed on the untreated glass surface over 72 h, while a slight increase in the number of cells is seen for the PEG films.
Original languageEnglish
Pages (from-to)444 - 448
Number of pages5
JournalSmall
Volume5
Issue number4
DOIs
Publication statusPublished - 2009
Externally publishedYes

Cite this