The preparation of low-fouling poly(ethylene glycol) (PEG) acrylate multilayers through the click-layer-by-layer (LbL) technique was reported. Radical polymerization was used to synthesize PEG-acrylate polymers with alkyne or azide functionalities. Nuclear magnetic resonance (NMR) spectroscopy show that PEG-Az contain approximately 15 azide groups and PEG-Alk 40 alkyne groups. It is also found that peptides can be coupled to the PEGylated surface by the attachment of GRGDSP-propargyl Gly, through analysis by ellipsometry. The potential of the PEG films to provide a low-biofouling surface assessed by comparing cell adhesion on untreated glass and PEG multilayers show that these cell surfaces have an elongated or flattened profile, exhibiting epithelial morphology. A steady growth of cells is observed on the untreated glass surface over 72 h, while a slight increase in the number of cells is seen for the PEG films.
Kinnane, C. R., Wark, K., Such, G. K., Johnston, A., & Caruso, F. (2009). Peptide-functionalized, low-biofouling click multilayers for promoting cell adhesion and growth. Small, 5(4), 444 - 448. https://doi.org/10.1002/smll.200801012