Peptide epitope binding and fluorescence quenching with the anti-mucin antibody C595

Martin J. Scanlon; Michael R. Price; Saul J B Tendler

doi:10.1016/0304-3835(92)90261-S

Peptide epitope binding and fluorescence quenching with the anti-mucin antibody C595

Martin J. Scanlon, Michael R. Price, Saul J B Tendler

Research output: Contribution to journal › Article › Research › peer-review

1 Citation (Scopus)

Abstract

The Fab fragment of the anti-polymorphic epithelial mucin antibody C595 (IgG3, κ) has been produced by digestion with papain. The purified antibody fragment retained its binding activity for the mucin in an indirect radioisotopic antiglobulin assay. The binding constant of the Fab fragment for the synthetic peptide (PDTRPAPGSTAPPAHGVTSA) corresponding to the 20-amino acid tandem repeat sequence found in the mucin protein core was determined to be 0.3 × 10⁶ M^-1 by measuring the capacity of the peptide to quench the fluorescence of the Fab fragment.

Original language	English
Pages (from-to)	199-202
Number of pages	4
Journal	Cancer Letters
Volume	63
Issue number	3
DOIs	https://doi.org/10.1016/0304-3835(92)90261-S
Publication status	Published - 30 Apr 1992
Externally published	Yes

Keywords

fluorescence quenching
mucin core related peptide
polymorphic epithelial mucins

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10.1016/0304-3835(92)90261-S

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Cite this

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title = "Peptide epitope binding and fluorescence quenching with the anti-mucin antibody C595",

abstract = "The Fab fragment of the anti-polymorphic epithelial mucin antibody C595 (IgG3, κ) has been produced by digestion with papain. The purified antibody fragment retained its binding activity for the mucin in an indirect radioisotopic antiglobulin assay. The binding constant of the Fab fragment for the synthetic peptide (PDTRPAPGSTAPPAHGVTSA) corresponding to the 20-amino acid tandem repeat sequence found in the mucin protein core was determined to be 0.3 × 106 M-1 by measuring the capacity of the peptide to quench the fluorescence of the Fab fragment.",

keywords = "fluorescence quenching, mucin core related peptide, polymorphic epithelial mucins",

author = "Scanlon, {Martin J.} and Price, {Michael R.} and Tendler, {Saul J B}",

year = "1992",

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T1 - Peptide epitope binding and fluorescence quenching with the anti-mucin antibody C595

AU - Scanlon, Martin J.

AU - Price, Michael R.

AU - Tendler, Saul J B

PY - 1992/4/30

Y1 - 1992/4/30

N2 - The Fab fragment of the anti-polymorphic epithelial mucin antibody C595 (IgG3, κ) has been produced by digestion with papain. The purified antibody fragment retained its binding activity for the mucin in an indirect radioisotopic antiglobulin assay. The binding constant of the Fab fragment for the synthetic peptide (PDTRPAPGSTAPPAHGVTSA) corresponding to the 20-amino acid tandem repeat sequence found in the mucin protein core was determined to be 0.3 × 106 M-1 by measuring the capacity of the peptide to quench the fluorescence of the Fab fragment.

AB - The Fab fragment of the anti-polymorphic epithelial mucin antibody C595 (IgG3, κ) has been produced by digestion with papain. The purified antibody fragment retained its binding activity for the mucin in an indirect radioisotopic antiglobulin assay. The binding constant of the Fab fragment for the synthetic peptide (PDTRPAPGSTAPPAHGVTSA) corresponding to the 20-amino acid tandem repeat sequence found in the mucin protein core was determined to be 0.3 × 106 M-1 by measuring the capacity of the peptide to quench the fluorescence of the Fab fragment.

KW - fluorescence quenching

KW - mucin core related peptide

KW - polymorphic epithelial mucins

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DO - 10.1016/0304-3835(92)90261-S

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VL - 63

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EP - 202

JO - Cancer Letters

JF - Cancer Letters

SN - 0304-3835

IS - 3

ER -