Peptide-based star polymers as potential siRNA carriers

Shu Jie Lam, Adrian Sulistio, Katharina Ladewig, Edgar Hoe Hon Wong, Anton Blencowe, Greg Qiao

Research output: Contribution to journalArticleResearchpeer-review

20 Citations (Scopus)

Abstract

16- and 32-arm star polymers were synthesised using poly(amido amine) (PAMAM) dendrimers as multifunctional initiators for the ring-opening polymerisation (ROP) of ε-Z-l-lysine N-carboxyanhydride (Lys NCA) via the core-first approach. The resulting star polymers were subsequently post-functionalised with poly(ethylene glycol) (PEG) via carbodiimide coupling, potentially improving the biodistribution of the stars in vivo. De-protection of the carboxybenzyl (Cbz)-protected star arms yielded water-soluble cationic poly(l-lysine) (PLL) star polymers with hydrodynamic radii ranging from 2.0 to 3.3nm. Successful complexation of the PLL star polymers with double-stranded oligodeoxynucleotides (ODNs) - a mimic for small interfering RNA (siRNA) - was achieved at a nitrogen-to-phosphate (N/P) ratio of 5. Cell viability studies using HEK293T cells indicated the 'safe' concentration for these polymers is within a suitable window for the delivery of siRNA therapeutics.

Original languageEnglish
Pages (from-to)592-597
Number of pages6
JournalAustralian Journal of Chemistry
Volume67
Issue number4
DOIs
Publication statusPublished - 2014
Externally publishedYes

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