Pentamycin Biosynthesis in Philippine Streptomyces sp. S816: Cytochrome P450-Catalyzed Installation of the C-14 Hydroxyl Group

Shanshan Zhou, Lijiang Song, Joleen Masschelein, Felaine A.M. Sumang, Irene A. Papa, Teofila O. Zulaybar, Aileen B. Custodio, Daniel Zabala, Edwin P. Alcantara, Emmanuel L.C. De Los Santos, Gregory L. Challis

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)

Abstract

Pentamycin is a polyene antibiotic, registered in Switzerland for the treatment of vaginal candidiasis, trichomoniasis, and mixed infections. Chemical instability has hindered its widespread application and development as a drug. Here, we report the identification of Streptomyces sp. S816, isolated from Philippine mangrove soil, as a pentamycin producer. Genome sequence analysis identified the putative pentamycin biosynthetic gene cluster, which shows a high degree of similarity to the gene cluster responsible for filipin III biosynthesis. The ptnJ gene, which is absent from the filipin III biosynthetic gene cluster, was shown to encode a cytochrome P450 capable of converting filipin III to pentamycin. This confirms that the cluster directs pentamycin biosynthesis, paving the way for biosynthetic engineering approaches to the production of pentamycin analogues. Several other Streptomyces genomes were found to contain ptnJ orthologues clustered with genes encoding polyketide synthases that appear to have similar architectures to those responsible for the assembly of filipin III and pentamycin, suggesting pentamycin production may be common in Streptomyces species.

Original languageEnglish
Pages (from-to)1305-1309
Number of pages5
JournalACS Chemical Biology
Volume14
Issue number6
DOIs
Publication statusPublished - 16 May 2019

Cite this