Patterned assembly of Yarrowia lipolytica yeast cells onto thermally evaporated octadecylamine films

Anand Gole, Vaishali Dixit, Neeta Lala, S. R. Sainkar, Aditi Pant, Murali Sastry

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)

Abstract

Thermally evaporated patterned films of the lipid, octadecylamine (ODA), have been used in the immobilization of the hydrocarbon-degrading cells, Yarrowia lipolytica. The immobilization of the yeast cells occurs on hydrophobic surfaces presented by the lipid film elements in the patterned structure, the attachment of the cells to the lipid film occurring possibly through hydrophobic interactions between the hydrocarbon chains of the fatty amine film and the cell wall of the yeasts. It is observed that the cell immobilization is extremely faithful to the underlying lipid template indicating potential use in tissue engineering as well as materials applications involving specific enzyme-based biotransformations.

Original languageEnglish
Pages (from-to)363-368
Number of pages6
JournalColloids and Surfaces B: Biointerfaces
Volume25
Issue number4
DOIs
Publication statusPublished - 1 Aug 2002
Externally publishedYes

Keywords

  • Cell attachment
  • Hydrophobic surface
  • Immobilization
  • Lipid bilayers
  • Quartz crystal microbalance

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