Patterned assembly of Yarrowia lipolytica yeast cells onto thermally evaporated octadecylamine films

Anand Gole; Vaishali Dixit; Neeta Lala; S. R. Sainkar; Aditi Pant; Murali Sastry

doi:10.1016/S0927-7765(02)00005-X

Patterned assembly of Yarrowia lipolytica yeast cells onto thermally evaporated octadecylamine films

Anand Gole, Vaishali Dixit, Neeta Lala, S. R. Sainkar, Aditi Pant, Murali Sastry

Research output: Contribution to journal › Article › Research › peer-review

9 Citations (Scopus)

Abstract

Thermally evaporated patterned films of the lipid, octadecylamine (ODA), have been used in the immobilization of the hydrocarbon-degrading cells, Yarrowia lipolytica. The immobilization of the yeast cells occurs on hydrophobic surfaces presented by the lipid film elements in the patterned structure, the attachment of the cells to the lipid film occurring possibly through hydrophobic interactions between the hydrocarbon chains of the fatty amine film and the cell wall of the yeasts. It is observed that the cell immobilization is extremely faithful to the underlying lipid template indicating potential use in tissue engineering as well as materials applications involving specific enzyme-based biotransformations.

Original language	English
Pages (from-to)	363-368
Number of pages	6
Journal	Colloids and Surfaces B: Biointerfaces
Volume	25
Issue number	4
DOIs	https://doi.org/10.1016/S0927-7765(02)00005-X
Publication status	Published - 1 Aug 2002
Externally published	Yes

Keywords

Cell attachment
Hydrophobic surface
Immobilization
Lipid bilayers
Quartz crystal microbalance

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10.1016/S0927-7765(02)00005-X

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title = "Patterned assembly of Yarrowia lipolytica yeast cells onto thermally evaporated octadecylamine films",

abstract = "Thermally evaporated patterned films of the lipid, octadecylamine (ODA), have been used in the immobilization of the hydrocarbon-degrading cells, Yarrowia lipolytica. The immobilization of the yeast cells occurs on hydrophobic surfaces presented by the lipid film elements in the patterned structure, the attachment of the cells to the lipid film occurring possibly through hydrophobic interactions between the hydrocarbon chains of the fatty amine film and the cell wall of the yeasts. It is observed that the cell immobilization is extremely faithful to the underlying lipid template indicating potential use in tissue engineering as well as materials applications involving specific enzyme-based biotransformations.",

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T1 - Patterned assembly of Yarrowia lipolytica yeast cells onto thermally evaporated octadecylamine films

AU - Gole, Anand

AU - Dixit, Vaishali

AU - Lala, Neeta

AU - Sainkar, S. R.

AU - Pant, Aditi

AU - Sastry, Murali

PY - 2002/8/1

Y1 - 2002/8/1

N2 - Thermally evaporated patterned films of the lipid, octadecylamine (ODA), have been used in the immobilization of the hydrocarbon-degrading cells, Yarrowia lipolytica. The immobilization of the yeast cells occurs on hydrophobic surfaces presented by the lipid film elements in the patterned structure, the attachment of the cells to the lipid film occurring possibly through hydrophobic interactions between the hydrocarbon chains of the fatty amine film and the cell wall of the yeasts. It is observed that the cell immobilization is extremely faithful to the underlying lipid template indicating potential use in tissue engineering as well as materials applications involving specific enzyme-based biotransformations.

AB - Thermally evaporated patterned films of the lipid, octadecylamine (ODA), have been used in the immobilization of the hydrocarbon-degrading cells, Yarrowia lipolytica. The immobilization of the yeast cells occurs on hydrophobic surfaces presented by the lipid film elements in the patterned structure, the attachment of the cells to the lipid film occurring possibly through hydrophobic interactions between the hydrocarbon chains of the fatty amine film and the cell wall of the yeasts. It is observed that the cell immobilization is extremely faithful to the underlying lipid template indicating potential use in tissue engineering as well as materials applications involving specific enzyme-based biotransformations.

KW - Cell attachment

KW - Hydrophobic surface

KW - Immobilization

KW - Lipid bilayers

KW - Quartz crystal microbalance

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U2 - 10.1016/S0927-7765(02)00005-X

DO - 10.1016/S0927-7765(02)00005-X

M3 - Article

AN - SCOPUS:0036703293

SN - 0927-7765

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JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

IS - 4

ER -

Patterned assembly of Yarrowia lipolytica yeast cells onto thermally evaporated octadecylamine films

Abstract

Keywords

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