Patient-administered tampon-collected genital cells in the assessment of Chlamydia trachomatis infection using polymerase chain reaction

Sepehr N. Tabrizi, Shujun Chen, Anthony J. Borg, Maxwell I. Lees, C. K. Fairley, Helen D. Jackson, Claudine H. Gust, Geoff Migliorini, Suzanne M. Garland

Research output: Contribution to journalArticleResearchpeer-review

17 Citations (Scopus)

Abstract

Background: Diagnosis of genital Chlamydia trachomatis infection in women traditionally requires a speculum examination to collect endocervical cells, followed by cell culture. This method is time consuming, requires stringent transport conditions, and is technically demanding. Goals: To compare tampons as a patient-administered collection method followed by detection with polymerase chain reaction (PCR) with the traditional endocervical swab culture followed by cell culture detection. Study Design: At the emergency department of a hospital for obstetrics and gynecology, 1,000 consecutive women with symptoms suggestive of infection with C. trachomatis were tested for C. trachomatis infection by PCR on both tampon (PCR-T) and swab (PCR-S) specimen and by culture of the swab specimen. Results: Seventeen PCR-T and 16 PCR-S specimens were positive; 16 endocervical specimens were positive by culture, and 14 of the endocervical samples were positive by the three methods. Sixty-one PCR-S samples were inadequate as shown by the lack of amplification of the β-globin gene segment, indicating poor collection of specimens by endocervical swab for chlamydial testing. Conclusions: Tampon specimens collected for PCR detection provided an easy and sensitive method of detection of C. trachomatis and overcame the obstacle of endocervical sampling and subsequent stringent transport requirements of culture.

Original languageEnglish
Pages (from-to)494-497
Number of pages4
JournalSexually Transmitted Diseases
Volume23
Issue number6
DOIs
Publication statusPublished - 1 Jan 1996

Cite this

Tabrizi, Sepehr N. ; Chen, Shujun ; Borg, Anthony J. ; Lees, Maxwell I. ; Fairley, C. K. ; Jackson, Helen D. ; Gust, Claudine H. ; Migliorini, Geoff ; Garland, Suzanne M. / Patient-administered tampon-collected genital cells in the assessment of Chlamydia trachomatis infection using polymerase chain reaction. In: Sexually Transmitted Diseases. 1996 ; Vol. 23, No. 6. pp. 494-497.
@article{dccee5bbe57f48158dc281acd7543027,
title = "Patient-administered tampon-collected genital cells in the assessment of Chlamydia trachomatis infection using polymerase chain reaction",
abstract = "Background: Diagnosis of genital Chlamydia trachomatis infection in women traditionally requires a speculum examination to collect endocervical cells, followed by cell culture. This method is time consuming, requires stringent transport conditions, and is technically demanding. Goals: To compare tampons as a patient-administered collection method followed by detection with polymerase chain reaction (PCR) with the traditional endocervical swab culture followed by cell culture detection. Study Design: At the emergency department of a hospital for obstetrics and gynecology, 1,000 consecutive women with symptoms suggestive of infection with C. trachomatis were tested for C. trachomatis infection by PCR on both tampon (PCR-T) and swab (PCR-S) specimen and by culture of the swab specimen. Results: Seventeen PCR-T and 16 PCR-S specimens were positive; 16 endocervical specimens were positive by culture, and 14 of the endocervical samples were positive by the three methods. Sixty-one PCR-S samples were inadequate as shown by the lack of amplification of the β-globin gene segment, indicating poor collection of specimens by endocervical swab for chlamydial testing. Conclusions: Tampon specimens collected for PCR detection provided an easy and sensitive method of detection of C. trachomatis and overcame the obstacle of endocervical sampling and subsequent stringent transport requirements of culture.",
author = "Tabrizi, {Sepehr N.} and Shujun Chen and Borg, {Anthony J.} and Lees, {Maxwell I.} and Fairley, {C. K.} and Jackson, {Helen D.} and Gust, {Claudine H.} and Geoff Migliorini and Garland, {Suzanne M.}",
year = "1996",
month = "1",
day = "1",
doi = "10.1097/00007435-199611000-00010",
language = "English",
volume = "23",
pages = "494--497",
journal = "Sexually Transmitted Diseases",
issn = "0148-5717",
publisher = "Lippincott Williams & Wilkins",
number = "6",

}

Patient-administered tampon-collected genital cells in the assessment of Chlamydia trachomatis infection using polymerase chain reaction. / Tabrizi, Sepehr N.; Chen, Shujun; Borg, Anthony J.; Lees, Maxwell I.; Fairley, C. K.; Jackson, Helen D.; Gust, Claudine H.; Migliorini, Geoff; Garland, Suzanne M.

In: Sexually Transmitted Diseases, Vol. 23, No. 6, 01.01.1996, p. 494-497.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Patient-administered tampon-collected genital cells in the assessment of Chlamydia trachomatis infection using polymerase chain reaction

AU - Tabrizi, Sepehr N.

AU - Chen, Shujun

AU - Borg, Anthony J.

AU - Lees, Maxwell I.

AU - Fairley, C. K.

AU - Jackson, Helen D.

AU - Gust, Claudine H.

AU - Migliorini, Geoff

AU - Garland, Suzanne M.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - Background: Diagnosis of genital Chlamydia trachomatis infection in women traditionally requires a speculum examination to collect endocervical cells, followed by cell culture. This method is time consuming, requires stringent transport conditions, and is technically demanding. Goals: To compare tampons as a patient-administered collection method followed by detection with polymerase chain reaction (PCR) with the traditional endocervical swab culture followed by cell culture detection. Study Design: At the emergency department of a hospital for obstetrics and gynecology, 1,000 consecutive women with symptoms suggestive of infection with C. trachomatis were tested for C. trachomatis infection by PCR on both tampon (PCR-T) and swab (PCR-S) specimen and by culture of the swab specimen. Results: Seventeen PCR-T and 16 PCR-S specimens were positive; 16 endocervical specimens were positive by culture, and 14 of the endocervical samples were positive by the three methods. Sixty-one PCR-S samples were inadequate as shown by the lack of amplification of the β-globin gene segment, indicating poor collection of specimens by endocervical swab for chlamydial testing. Conclusions: Tampon specimens collected for PCR detection provided an easy and sensitive method of detection of C. trachomatis and overcame the obstacle of endocervical sampling and subsequent stringent transport requirements of culture.

AB - Background: Diagnosis of genital Chlamydia trachomatis infection in women traditionally requires a speculum examination to collect endocervical cells, followed by cell culture. This method is time consuming, requires stringent transport conditions, and is technically demanding. Goals: To compare tampons as a patient-administered collection method followed by detection with polymerase chain reaction (PCR) with the traditional endocervical swab culture followed by cell culture detection. Study Design: At the emergency department of a hospital for obstetrics and gynecology, 1,000 consecutive women with symptoms suggestive of infection with C. trachomatis were tested for C. trachomatis infection by PCR on both tampon (PCR-T) and swab (PCR-S) specimen and by culture of the swab specimen. Results: Seventeen PCR-T and 16 PCR-S specimens were positive; 16 endocervical specimens were positive by culture, and 14 of the endocervical samples were positive by the three methods. Sixty-one PCR-S samples were inadequate as shown by the lack of amplification of the β-globin gene segment, indicating poor collection of specimens by endocervical swab for chlamydial testing. Conclusions: Tampon specimens collected for PCR detection provided an easy and sensitive method of detection of C. trachomatis and overcame the obstacle of endocervical sampling and subsequent stringent transport requirements of culture.

UR - http://www.scopus.com/inward/record.url?scp=0029850275&partnerID=8YFLogxK

U2 - 10.1097/00007435-199611000-00010

DO - 10.1097/00007435-199611000-00010

M3 - Article

C2 - 8946635

AN - SCOPUS:0029850275

VL - 23

SP - 494

EP - 497

JO - Sexually Transmitted Diseases

JF - Sexually Transmitted Diseases

SN - 0148-5717

IS - 6

ER -