Patch-clamp recordings from the soma and dendrites of neurons in brain slices using infrared video microscopy

G. J. Stuart; H. U. Dodt; B. Sakmann

doi:10.1007/BF00374949

Patch-clamp recordings from the soma and dendrites of neurons in brain slices using infrared video microscopy

G. J. Stuart, H. U. Dodt, B. Sakmann

Research output: Contribution to journal › Article › Research › peer-review

654 Citations (Scopus)

Abstract

A description is given of the implementation of infrared differential interference contrast (IR-DIC) video microscopy to an upright compound microscope. Using the improved resolution offered by IR-DIC a procedure is described for making patch-pipette recordings from visually identified neuronal somata and dendrites in brain slices. As an example of the application of this technique to electrophysiological recordings from small neuronal processes in brain slices we describe wholecell current-clamp and cell-attached and excised patchclamp recordings from the apical dendrites of layer V pyramidal neurons in slices of rat neocortex.

Original language	English
Pages (from-to)	511-518
Number of pages	8
Journal	Pflügers Archiv European Journal of Physiology
Volume	423
Issue number	5-6
DOIs	https://doi.org/10.1007/BF00374949
Publication status	Published - Jun 1993
Externally published	Yes

Keywords

Brain slice
Dendrite
Neocortex
Patch clamp
Video microscopy

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10.1007/BF00374949

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KW - Dendrite

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Patch-clamp recordings from the soma and dendrites of neurons in brain slices using infrared video microscopy

Abstract

Keywords

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