Passaged human chondrocytes accumulate extracellular matrix when induced by bovine chondrocytes

Nazish Ahmed, Drew W. Taylor, Jay Wunder, Andras Nagy, Allan E. Gross, Rita A. Kandel

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21 Citations (Scopus)

Abstract

A source of sufficient number of cells is a major limiting factor for cartilage tissue engineering. To circumvent this problem, we developed a co-culture method to induce redifferentiation in bovine articular chondrocytes, which had undergone dedifferentiation following serial passage in monolayer culture. In this study we determine whether human osteoarthritic (OA) and non-diseased passaged dedifferentiated chondrocytes will respond similarly. Human passaged chondrocytes were co-cultured for 4 weeks with primary bovine chondrocytes and their redifferentiation status was determined. Afterwards the cells were cultured either independently or in coculture with cryopreserved passaged cells for functional analysis. The co-culture of passaged cells with primary chondrocytes resulted in reversion of their phenotype towards articular chondrocytes, as shown by increased gene expression of type II collagen and COMP, decreased type I collagen expression and extracellular matrix formation in vitro. Furthermore, this redifferentiation was stable, as those cells not only formed hyaline-like cartilage tissue when grown on their own but also they could induce redifferentiation of passaged chondrocytes in co-culture. These data suggest that it may be possible to use autologous chondrocytes obtained from osteoarthritic cartilage to form tissue suitable to use for cartilage repair.

Original languageEnglish
Pages (from-to)233-241
Number of pages9
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume4
Issue number3
DOIs
Publication statusPublished - Mar 2010
Externally publishedYes

Keywords

  • Chondrocytes
  • Co-culture
  • Osteoarthritis
  • Redifferentiation
  • Tissue regeneration

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