Projects per year
Abstract
Growth factor receptor bound protein 7 (Grb7) is a mammalian adaptor protein participating in signaling pathways implicated in cell migration, metastatic invasion, cell proliferation and tumor-associated angiogenesis. We expressed tagged versions of wild type Grb7 and the mutant Grb7Δ, lacking its calmodulin-binding domain (CaM-BD), in human embryonic kidney (HEK) 293 cells and rat glioma C6 cells to identify novel binding partners using shot-gun proteomics. Among the new identified proteins, we validated the ubiquitin-ligase Nedd4 (neural precursor cell expressed developmentally down-regulated protein 4), the heat-shock protein Hsc70/HSPA8 (heat shock cognate protein 70) and the cell cycle regulatory protein caprin-1 (cytoplasmic activation/proliferation-associated protein 1) in rat glioma C6 cells. Our results suggest a role of Grb7 in pathways where these proteins are implicated. These include protein trafficking and degradation, stress-response, chaperone-mediated autophagy, apoptosis and cell proliferation.
Original language | English |
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Article number | 108386 |
Number of pages | 8 |
Journal | Archives of Biochemistry and Biophysics |
Volume | 687 |
DOIs | |
Publication status | Published - 15 Jul 2020 |
Keywords
- Calmodulin
- Caprin-1
- Grb7
- Hsc70
- Nedd4
- Proteomics
Projects
- 1 Finished
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Design and delivery of peptide-based anti-cancer Grb7 inhibitors
Wilce, J., Perlmutter, P. & Pero, S.
National Health and Medical Research Council (NHMRC) (Australia)
1/01/13 → 30/06/16
Project: Research