Parathyroid hormone-related protein purified from a human lung cancer cell line

J. M. Moseley, M. Kubota, H. Diefenbach-Jagger, R. E. Wettenhall, B. E. Kemp, L. J. Suva, C. P. Rodda, P. R. Ebeling, P. J. Hudson, J. D. Zajac

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A protein with biological activities similar to parathyroid hormone (PTH) has been purified from serum-free culture medium obtained from a human lung cancer cell line (BEN). A major protein band of 18 kDa was obtained on NaDodSO4/polyacrylamide gels, with faint bands at 35 kDa and 67 kDa. Biological activity was associated only with the 18-kDa band. Amino acid sequence analysis of the material purified by HPLC revealed that 8 of the 16 residues were identical with those of human PTH. Antibody raised to a corresponding synthetic peptide recognized the PTH-related material but showed < 1% cross-reactivity with human PTH amino-terminal peptides. BEN cells contained PTH DNA, but not PTH messenger RNA, indicating involvement of another gene. The purified PTH-related protein had a specific biological activity ≃ 6 times greater than that of bovine PTH(1-34). The PTH-related protein may have a role in the syndrome of humoral hypercalcemia of malignancy.

Original languageEnglish
Pages (from-to)5048-5052
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number14
Publication statusPublished - 1 Sep 1987
Externally publishedYes

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