Pancreas differentiation of mouse ES cells.

Suzanne Jeanine Micallef, Xueling Li, Andrew George Elefanty, Edouard Stanley

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    This unit describes the derivation of pancreatic cells from mouse embryonic stem cells (ESCs). Mouse ESCs are pluripotent immortal cells derived from the inner cell mass of pre-implantation blastocyst-stage embryos that possess the ability to differentiate into any cell type within the adult animal. In vitro, ESCs can be differentiated into a variety of cell types representing derivatives of the three embryonic germ layers, mesoderm, endoderm, and ectoderm. Successfully differentiating ES cells to pancreatic cells has the potential to provide an alternative to cadaver-derived cells for treatment of type I diabetes. This unit outlines a method for the differentiation of ESCs toward pancreatic endoderm in serum-free medium from embryoid bodies (EBs) formed in suspension or spin EBs. In addition there is a protocol for maintaining ESC.
    Original languageEnglish
    Pages (from-to)G2.1 - G2.8
    Number of pages8
    JournalCurrent Protocols in Stem Cell Biology
    VolumeChapter 1
    Issue numberIG.2
    Publication statusPublished - 2007

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