TY - JOUR
T1 - Palm gamma-tocotrienol supplementation suppress tumour growth and metastasis in a syngeneic mouse model of breast cancer
AU - Subramaniam, Shonia
AU - Radhakrishnan, Ammu Kutty
AU - Anandha Rao, Jeya Seela
AU - Ramdas, Premdass
AU - Han, Ng Mei
AU - Kutty, Methil Kannan
AU - Selvaduray, Kanga Rani
N1 - Funding Information:
The authors would like to thank the International Medical University for providing the research facilities to carry out this study. This work was supported by a research grant from the Malaysian Palm Oil Board (MPOB) [PD167] and an FRGS grant (FRGS/1/2013/SG05/IMU/01/1). All the lab work carried out at International Medical University (IMU).
Funding Information:
The authors would like to thank the International Medical University for providing the research facilities to carry out this study. This work was supported by a research grant from the Malaysian Palm?Oil?Board?(MPOB)?[PD167]?and?an?FRGS?grant? (FRGS/1/2013/SG05/IMU/01/1). All the lab work carried out at International Medical University (IMU).
Publisher Copyright:
© 2022 Lembaga Minyak Sawit Malaysia. All rights reserved.
PY - 2022/6
Y1 - 2022/6
N2 - Gamma-tocotrienol (γT3) is an isoform of vitamin E found abundantly in palm oil, which is reported to possess antioxidant and anticancer activities. However, the immune-modulating properties of γT3 have yet to be elucidated. Breast cancer (BC) was induced in female BALB/c mice by injecting 4T1 murine mammary cancer cells into their mammary fat pads. When the tumour was palpable, the animals were randomly assigned into two groups: (i) control [fed twice daily with 50 μL vehicle (soy oil)] or (ii) experimental (fed twice daily with 50 μL of 0.5 mg of γT3). Results show that mice fed with γT3 had reduced tumour growth and metastasis. However, there are no marked changes in the percentages of peripheral blood leukocytes and cytokines production in these animals. Immunohistochemistry using antibodies to murine CD4, IL12Rβ2, IL24 and FoxP3 on tumour sections from γT3 treated mice suggested that γT3 induced suppression of tumour growth and metastasis as well as reduced immunosuppression in the tumour microenvironment. It can be concluded that, γT3 has the potential to suppress tumour growth and metastasis in this model. Further investigation on the host immune response is possible by prolonging treatment duration against BC.
AB - Gamma-tocotrienol (γT3) is an isoform of vitamin E found abundantly in palm oil, which is reported to possess antioxidant and anticancer activities. However, the immune-modulating properties of γT3 have yet to be elucidated. Breast cancer (BC) was induced in female BALB/c mice by injecting 4T1 murine mammary cancer cells into their mammary fat pads. When the tumour was palpable, the animals were randomly assigned into two groups: (i) control [fed twice daily with 50 μL vehicle (soy oil)] or (ii) experimental (fed twice daily with 50 μL of 0.5 mg of γT3). Results show that mice fed with γT3 had reduced tumour growth and metastasis. However, there are no marked changes in the percentages of peripheral blood leukocytes and cytokines production in these animals. Immunohistochemistry using antibodies to murine CD4, IL12Rβ2, IL24 and FoxP3 on tumour sections from γT3 treated mice suggested that γT3 induced suppression of tumour growth and metastasis as well as reduced immunosuppression in the tumour microenvironment. It can be concluded that, γT3 has the potential to suppress tumour growth and metastasis in this model. Further investigation on the host immune response is possible by prolonging treatment duration against BC.
KW - 4T1
KW - breast cancer
KW - gamma-tocotrienol
KW - mouse
KW - supplementation
UR - http://www.scopus.com/inward/record.url?scp=85141513028&partnerID=8YFLogxK
U2 - 10.21894/jopr.2021.0048
DO - 10.21894/jopr.2021.0048
M3 - Article
AN - SCOPUS:85141513028
SN - 1511-2780
VL - 34
SP - 368
EP - 379
JO - Journal of Oil Palm Research
JF - Journal of Oil Palm Research
IS - 2
ER -
