Abstract
Using a variety of peptide analogues of oxytocin (OT) and Arg8-vasopressin (AVP), OT-mediated induction of urokinase-type plasminogen activator (uPA) was examined in LLC-PK1 renal epithelial cells, which possess distinct high-affinity receptors of both the OT- and vasopressin renal (V2-) types. OT or OT-receptor specific agonists induced concentration-dependent cAMP synthesis, activation of the cAMP-dependent protein kinase (cAMP-PK) and uPA production consistent with their respective binding affinities for the V2- and not the OT-receptor. OT-mediated uPA induction could be inhibited in a concentration-dependent fashion by coincubation with a V2/V1-receptor specific antagonist, but not by an OT-receptor specific antagonist. Results implied that stimulation of cAMP- and uPA responses in LLC-PK1 cells by OT was V2-receptor-mediated.
Original language | English |
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Pages (from-to) | 134-138 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 315 |
Issue number | 2 |
DOIs | |
Publication status | Published - 4 Jan 1993 |
Externally published | Yes |
Keywords
- Neurohypophyseal nonapeptide hormone
- Specific antagonist
- Urokinase-type plasminogen activator
- Vasopressin V-receptor