Oxygen, temperature and humidity of inspired gases and their influences on airway and lung tissue in near-term lambs

Jane Jane Pillow, Noah H Hillman, Graeme R Polglase, Timothy J M Moss, Suhas G Kallapur, Fook-Choe Cheah, Boris W Kramer, Alan H Jobe

Research output: Contribution to journalArticleResearchpeer-review

Abstract

PURPOSE: The relative contributions of factors influencing lung injury immediately after birth are poorly understood. We hypothesized that oxygen content and humidity of inspired air would influence markers of pulmonary inflammation in ventilated lambs. METHODS: Lambs delivered at 140 days gestation (term = 150 days) were assigned to one of five groups (n = 5-6/group): unventilated controls, or ventilation with 21 or 100 O(2) that was either heated and humidified or cold and dry. Lambs were ventilated gently for 3 h: blood gases were recorded regularly. Bronchoalveolar lavage and samples of tracheal mucosa and lung were collected post mortem. RESULTS: Arterial pH was lower [mean difference (95 CI): -0.07 (-0.13, -0.03)], while there was an increase in PaCO(2) [mean difference (95 CI): 10.2 (2.4, 17.9)] and fold change in lung pro-inflammatory IL-1beta cytokine mRNA [mean difference (95 CI): 28.3 (0.3, 56.2)] or IL-8 [mean difference (95 CI): 27.8 (7.9, 47.7)] cytokine mRNA expression with 100 O(2) relative to 21 O(2). Cold dry inspired gas did not influence gas exchange or dynamic mechanics at 3 h compared to heated humidified gas. Compared to 100 inspired O(2), cold dry inspired gas had less marked effect on fold change in lung pro-inflammatory IL-1beta cytokine mRNA [mean difference (95 CI): 27.2 (-0/8, 55.1)] or IL-8 [mean difference (95 CI): 14.5 (5.5, 34.4)] cytokine mRNA expression, although cilial dysfunction/damage was evident on electron microscopy, especially when exposure to cold dry gas was combined with hyperoxia. CONCLUSIONS: In near-term neonatal lambs ventilated for 3 h, hyperoxia was associated a more powerful stimulus for pulmonary dysfunction and upregulation of inflammatory cytokines than cold dry gas.
Original languageEnglish
Pages (from-to)2157 - 2163
Number of pages7
JournalIntensive Care Medicine
Volume35
Issue number12
DOIs
Publication statusPublished - 2009

Cite this

Pillow, Jane Jane ; Hillman, Noah H ; Polglase, Graeme R ; Moss, Timothy J M ; Kallapur, Suhas G ; Cheah, Fook-Choe ; Kramer, Boris W ; Jobe, Alan H. / Oxygen, temperature and humidity of inspired gases and their influences on airway and lung tissue in near-term lambs. In: Intensive Care Medicine. 2009 ; Vol. 35, No. 12. pp. 2157 - 2163.
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title = "Oxygen, temperature and humidity of inspired gases and their influences on airway and lung tissue in near-term lambs",
abstract = "PURPOSE: The relative contributions of factors influencing lung injury immediately after birth are poorly understood. We hypothesized that oxygen content and humidity of inspired air would influence markers of pulmonary inflammation in ventilated lambs. METHODS: Lambs delivered at 140 days gestation (term = 150 days) were assigned to one of five groups (n = 5-6/group): unventilated controls, or ventilation with 21 or 100 O(2) that was either heated and humidified or cold and dry. Lambs were ventilated gently for 3 h: blood gases were recorded regularly. Bronchoalveolar lavage and samples of tracheal mucosa and lung were collected post mortem. RESULTS: Arterial pH was lower [mean difference (95 CI): -0.07 (-0.13, -0.03)], while there was an increase in PaCO(2) [mean difference (95 CI): 10.2 (2.4, 17.9)] and fold change in lung pro-inflammatory IL-1beta cytokine mRNA [mean difference (95 CI): 28.3 (0.3, 56.2)] or IL-8 [mean difference (95 CI): 27.8 (7.9, 47.7)] cytokine mRNA expression with 100 O(2) relative to 21 O(2). Cold dry inspired gas did not influence gas exchange or dynamic mechanics at 3 h compared to heated humidified gas. Compared to 100 inspired O(2), cold dry inspired gas had less marked effect on fold change in lung pro-inflammatory IL-1beta cytokine mRNA [mean difference (95 CI): 27.2 (-0/8, 55.1)] or IL-8 [mean difference (95 CI): 14.5 (5.5, 34.4)] cytokine mRNA expression, although cilial dysfunction/damage was evident on electron microscopy, especially when exposure to cold dry gas was combined with hyperoxia. CONCLUSIONS: In near-term neonatal lambs ventilated for 3 h, hyperoxia was associated a more powerful stimulus for pulmonary dysfunction and upregulation of inflammatory cytokines than cold dry gas.",
author = "Pillow, {Jane Jane} and Hillman, {Noah H} and Polglase, {Graeme R} and Moss, {Timothy J M} and Kallapur, {Suhas G} and Fook-Choe Cheah and Kramer, {Boris W} and Jobe, {Alan H}",
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Oxygen, temperature and humidity of inspired gases and their influences on airway and lung tissue in near-term lambs. / Pillow, Jane Jane; Hillman, Noah H; Polglase, Graeme R; Moss, Timothy J M; Kallapur, Suhas G; Cheah, Fook-Choe; Kramer, Boris W; Jobe, Alan H.

In: Intensive Care Medicine, Vol. 35, No. 12, 2009, p. 2157 - 2163.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Oxygen, temperature and humidity of inspired gases and their influences on airway and lung tissue in near-term lambs

AU - Pillow, Jane Jane

AU - Hillman, Noah H

AU - Polglase, Graeme R

AU - Moss, Timothy J M

AU - Kallapur, Suhas G

AU - Cheah, Fook-Choe

AU - Kramer, Boris W

AU - Jobe, Alan H

PY - 2009

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N2 - PURPOSE: The relative contributions of factors influencing lung injury immediately after birth are poorly understood. We hypothesized that oxygen content and humidity of inspired air would influence markers of pulmonary inflammation in ventilated lambs. METHODS: Lambs delivered at 140 days gestation (term = 150 days) were assigned to one of five groups (n = 5-6/group): unventilated controls, or ventilation with 21 or 100 O(2) that was either heated and humidified or cold and dry. Lambs were ventilated gently for 3 h: blood gases were recorded regularly. Bronchoalveolar lavage and samples of tracheal mucosa and lung were collected post mortem. RESULTS: Arterial pH was lower [mean difference (95 CI): -0.07 (-0.13, -0.03)], while there was an increase in PaCO(2) [mean difference (95 CI): 10.2 (2.4, 17.9)] and fold change in lung pro-inflammatory IL-1beta cytokine mRNA [mean difference (95 CI): 28.3 (0.3, 56.2)] or IL-8 [mean difference (95 CI): 27.8 (7.9, 47.7)] cytokine mRNA expression with 100 O(2) relative to 21 O(2). Cold dry inspired gas did not influence gas exchange or dynamic mechanics at 3 h compared to heated humidified gas. Compared to 100 inspired O(2), cold dry inspired gas had less marked effect on fold change in lung pro-inflammatory IL-1beta cytokine mRNA [mean difference (95 CI): 27.2 (-0/8, 55.1)] or IL-8 [mean difference (95 CI): 14.5 (5.5, 34.4)] cytokine mRNA expression, although cilial dysfunction/damage was evident on electron microscopy, especially when exposure to cold dry gas was combined with hyperoxia. CONCLUSIONS: In near-term neonatal lambs ventilated for 3 h, hyperoxia was associated a more powerful stimulus for pulmonary dysfunction and upregulation of inflammatory cytokines than cold dry gas.

AB - PURPOSE: The relative contributions of factors influencing lung injury immediately after birth are poorly understood. We hypothesized that oxygen content and humidity of inspired air would influence markers of pulmonary inflammation in ventilated lambs. METHODS: Lambs delivered at 140 days gestation (term = 150 days) were assigned to one of five groups (n = 5-6/group): unventilated controls, or ventilation with 21 or 100 O(2) that was either heated and humidified or cold and dry. Lambs were ventilated gently for 3 h: blood gases were recorded regularly. Bronchoalveolar lavage and samples of tracheal mucosa and lung were collected post mortem. RESULTS: Arterial pH was lower [mean difference (95 CI): -0.07 (-0.13, -0.03)], while there was an increase in PaCO(2) [mean difference (95 CI): 10.2 (2.4, 17.9)] and fold change in lung pro-inflammatory IL-1beta cytokine mRNA [mean difference (95 CI): 28.3 (0.3, 56.2)] or IL-8 [mean difference (95 CI): 27.8 (7.9, 47.7)] cytokine mRNA expression with 100 O(2) relative to 21 O(2). Cold dry inspired gas did not influence gas exchange or dynamic mechanics at 3 h compared to heated humidified gas. Compared to 100 inspired O(2), cold dry inspired gas had less marked effect on fold change in lung pro-inflammatory IL-1beta cytokine mRNA [mean difference (95 CI): 27.2 (-0/8, 55.1)] or IL-8 [mean difference (95 CI): 14.5 (5.5, 34.4)] cytokine mRNA expression, although cilial dysfunction/damage was evident on electron microscopy, especially when exposure to cold dry gas was combined with hyperoxia. CONCLUSIONS: In near-term neonatal lambs ventilated for 3 h, hyperoxia was associated a more powerful stimulus for pulmonary dysfunction and upregulation of inflammatory cytokines than cold dry gas.

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