Oxygen-permeable microwell device maintains islet mass and integrity during shipping

Darling M. Rojas-Canales, Michaela Waibel, Aurelien Forget, Daniella Penko, Jodie Nitschke, Fran J. Harding, Bahman Delalat, Anton Blencowe, Thomas Loudovaris, Shane T. Grey, Helen E. Thomas, Thomas W.H. Kay, Chris J. Drogemuller, Nicolas H. Voelcker, Patrick T. Coates

Research output: Contribution to journalArticleResearchpeer-review

1 Citation (Scopus)

Abstract

Islet transplantation is currently the only minimally invasive therapy available for patients with type 1 diabetes that can lead to insulin independence; however, it is limited to only a small number of patients. Although clinical procedures have improved in the isolation and culture of islets, a large number of islets are still lost in the pre-transplant period, limiting the success of this treatment. Moreover, current practice includes islets being prepared at specialized centers, which are sometimes remote to the transplant location. Thus, a critical point of intervention to maintain the quality and quantity of isolated islets is during transportation between isolation centers and the transplanting hospitals, during which 20–40% of functional islets can be lost. The current study investigated the use of an oxygen-permeable PDMS microwell device for long-distance transportation of isolated islets. We demonstrate that the microwell device protected islets from aggregation during transport, maintaining viability and average islet size during shipping.

Original languageEnglish
Pages (from-to)490-503
Number of pages14
JournalEndocrine Connections
Volume7
Issue number3
DOIs
Publication statusPublished - 1 Mar 2018

Keywords

  • Hypoxia
  • Islet
  • Microwell
  • Shipping
  • Transplantation

Cite this

Rojas-Canales, D. M., Waibel, M., Forget, A., Penko, D., Nitschke, J., Harding, F. J., ... Coates, P. T. (2018). Oxygen-permeable microwell device maintains islet mass and integrity during shipping. Endocrine Connections, 7(3), 490-503. https://doi.org/10.1530/EC-17-0349
Rojas-Canales, Darling M. ; Waibel, Michaela ; Forget, Aurelien ; Penko, Daniella ; Nitschke, Jodie ; Harding, Fran J. ; Delalat, Bahman ; Blencowe, Anton ; Loudovaris, Thomas ; Grey, Shane T. ; Thomas, Helen E. ; Kay, Thomas W.H. ; Drogemuller, Chris J. ; Voelcker, Nicolas H. ; Coates, Patrick T. / Oxygen-permeable microwell device maintains islet mass and integrity during shipping. In: Endocrine Connections. 2018 ; Vol. 7, No. 3. pp. 490-503.
@article{cf6be69dbf684001a340188be47961d9,
title = "Oxygen-permeable microwell device maintains islet mass and integrity during shipping",
abstract = "Islet transplantation is currently the only minimally invasive therapy available for patients with type 1 diabetes that can lead to insulin independence; however, it is limited to only a small number of patients. Although clinical procedures have improved in the isolation and culture of islets, a large number of islets are still lost in the pre-transplant period, limiting the success of this treatment. Moreover, current practice includes islets being prepared at specialized centers, which are sometimes remote to the transplant location. Thus, a critical point of intervention to maintain the quality and quantity of isolated islets is during transportation between isolation centers and the transplanting hospitals, during which 20–40{\%} of functional islets can be lost. The current study investigated the use of an oxygen-permeable PDMS microwell device for long-distance transportation of isolated islets. We demonstrate that the microwell device protected islets from aggregation during transport, maintaining viability and average islet size during shipping.",
keywords = "Hypoxia, Islet, Microwell, Shipping, Transplantation",
author = "Rojas-Canales, {Darling M.} and Michaela Waibel and Aurelien Forget and Daniella Penko and Jodie Nitschke and Harding, {Fran J.} and Bahman Delalat and Anton Blencowe and Thomas Loudovaris and Grey, {Shane T.} and Thomas, {Helen E.} and Kay, {Thomas W.H.} and Drogemuller, {Chris J.} and Voelcker, {Nicolas H.} and Coates, {Patrick T.}",
year = "2018",
month = "3",
day = "1",
doi = "10.1530/EC-17-0349",
language = "English",
volume = "7",
pages = "490--503",
journal = "Endocrine Connections",
issn = "2049-3614",
publisher = "Bioscientifica",
number = "3",

}

Rojas-Canales, DM, Waibel, M, Forget, A, Penko, D, Nitschke, J, Harding, FJ, Delalat, B, Blencowe, A, Loudovaris, T, Grey, ST, Thomas, HE, Kay, TWH, Drogemuller, CJ, Voelcker, NH & Coates, PT 2018, 'Oxygen-permeable microwell device maintains islet mass and integrity during shipping', Endocrine Connections, vol. 7, no. 3, pp. 490-503. https://doi.org/10.1530/EC-17-0349

Oxygen-permeable microwell device maintains islet mass and integrity during shipping. / Rojas-Canales, Darling M.; Waibel, Michaela; Forget, Aurelien; Penko, Daniella; Nitschke, Jodie; Harding, Fran J.; Delalat, Bahman; Blencowe, Anton; Loudovaris, Thomas; Grey, Shane T.; Thomas, Helen E.; Kay, Thomas W.H.; Drogemuller, Chris J.; Voelcker, Nicolas H.; Coates, Patrick T.

In: Endocrine Connections, Vol. 7, No. 3, 01.03.2018, p. 490-503.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Oxygen-permeable microwell device maintains islet mass and integrity during shipping

AU - Rojas-Canales, Darling M.

AU - Waibel, Michaela

AU - Forget, Aurelien

AU - Penko, Daniella

AU - Nitschke, Jodie

AU - Harding, Fran J.

AU - Delalat, Bahman

AU - Blencowe, Anton

AU - Loudovaris, Thomas

AU - Grey, Shane T.

AU - Thomas, Helen E.

AU - Kay, Thomas W.H.

AU - Drogemuller, Chris J.

AU - Voelcker, Nicolas H.

AU - Coates, Patrick T.

PY - 2018/3/1

Y1 - 2018/3/1

N2 - Islet transplantation is currently the only minimally invasive therapy available for patients with type 1 diabetes that can lead to insulin independence; however, it is limited to only a small number of patients. Although clinical procedures have improved in the isolation and culture of islets, a large number of islets are still lost in the pre-transplant period, limiting the success of this treatment. Moreover, current practice includes islets being prepared at specialized centers, which are sometimes remote to the transplant location. Thus, a critical point of intervention to maintain the quality and quantity of isolated islets is during transportation between isolation centers and the transplanting hospitals, during which 20–40% of functional islets can be lost. The current study investigated the use of an oxygen-permeable PDMS microwell device for long-distance transportation of isolated islets. We demonstrate that the microwell device protected islets from aggregation during transport, maintaining viability and average islet size during shipping.

AB - Islet transplantation is currently the only minimally invasive therapy available for patients with type 1 diabetes that can lead to insulin independence; however, it is limited to only a small number of patients. Although clinical procedures have improved in the isolation and culture of islets, a large number of islets are still lost in the pre-transplant period, limiting the success of this treatment. Moreover, current practice includes islets being prepared at specialized centers, which are sometimes remote to the transplant location. Thus, a critical point of intervention to maintain the quality and quantity of isolated islets is during transportation between isolation centers and the transplanting hospitals, during which 20–40% of functional islets can be lost. The current study investigated the use of an oxygen-permeable PDMS microwell device for long-distance transportation of isolated islets. We demonstrate that the microwell device protected islets from aggregation during transport, maintaining viability and average islet size during shipping.

KW - Hypoxia

KW - Islet

KW - Microwell

KW - Shipping

KW - Transplantation

UR - http://www.scopus.com/inward/record.url?scp=85052317401&partnerID=8YFLogxK

U2 - 10.1530/EC-17-0349

DO - 10.1530/EC-17-0349

M3 - Article

AN - SCOPUS:85052317401

VL - 7

SP - 490

EP - 503

JO - Endocrine Connections

JF - Endocrine Connections

SN - 2049-3614

IS - 3

ER -

Rojas-Canales DM, Waibel M, Forget A, Penko D, Nitschke J, Harding FJ et al. Oxygen-permeable microwell device maintains islet mass and integrity during shipping. Endocrine Connections. 2018 Mar 1;7(3):490-503. https://doi.org/10.1530/EC-17-0349