Oxidative stress-induced insulin resistance in skeletal muscle cells is ameliorated by gamma-tocopherol treatment

Indu Singh, Andrew L. Carey, Nadine Watson, Mark A. Febbraio, John A. Hawley

Research output: Contribution to journalArticleResearchpeer-review

30 Citations (Scopus)

Abstract

Background: Oxidative stress-induced reactive oxygen species are associated with the clinical manifestation of insulin resistance. Evidence suggests that antioxidant treatment may reduce this incidence. Aim of the study: This study determined whether glucose oxidase (GO)-induced insulin resistance in cultured skeletal muscle cells could be ameliorated by pre-treatment with gamma-tocopherol (GT). Methods: Insulin sensitivity in L6 myotubes was assessed by 2-deoxy-d-[3H]-glucose uptake. The phosphorylation of distal insulin signaling proteins Akt and the Akt substrate AS160 were determined by western blot. Results: One hour treatment with 100 mU/ml GO decreased insulin-stimulated glucose uptake (P < 0.001). Pre-treatment with GT either partially (100 μM) or completely (200 μM) restored insulin-stimulated glucose uptake in cells after GO-induced insulin resistance. GO-induced oxidative stress did not impair insulin stimulated phosphorylation of Akt or AS160, but 200 μM GT increased insulin-stimulated phosphorylation of these key signaling proteins (P < 0.05). Conclusions: High-dose (200 μM) GT treatment ameliorated oxidative stress-induced insulin resistance in cultured rat L6 skeletal muscle cells.

Original languageEnglish
Pages (from-to)387-392
Number of pages6
JournalEuropean Journal of Nutrition
Volume47
Issue number7
DOIs
Publication statusPublished - 1 Oct 2008
Externally publishedYes

Keywords

  • Akt
  • Antioxidants
  • AS160
  • Glucose oxidase
  • Glucose transport
  • L6 myotubes

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