TY - JOUR
T1 - Overlapping T-cell epitopes in the group I allergen of Dermatophagoides species restricted by HLA-DP and HLA-DR class II molecules
AU - Higgins, Julie A.
AU - Thorpe, Christopher J.
AU - Hayball, John D.
AU - O'Hehir, Robyn E.
AU - Lamb, Jonathan R.
PY - 1994/1/1
Y1 - 1994/1/1
N2 - The induction of IgE antibodies reactive with the group I allergen of Dermatophagoides species (house dust mite [HDM]), which comprise a major component of the allergic immune response in HDM-atopic individuals, is dependent on the functional activity of specific CD4 + T cells. In this report we demonstrate that for a particular HDM-atopic individual the T-cell response to the group I allergen of Dermatophagoides pteronyssinus (Der p I) is limited to a single region (residues 101-143) of the protein. By mapping the fine antigen specificity with T-cell clones, we observed that the sequence 101-131 of Der p I contains a cluster of at least three overlapping T-cell epitopes. Analysis of the HLA class II restriction specificity of the T-cell clones revealed that the T-cell epitope, residues 110-131, was restricted by HLA-DRB1 *0101. In contrast, peptide Der p 1, 110-119 was recognized in association with HLA-DPB1 *0402. However, the ability of cloned T cells to proliferate to the peptide Der p 1, 107-119 presented by HLA-DPB1 *0401, HLA-DPB1 *0402, and HLA-DPB1 *0501 expressing accessory cells illustrates the heterogeneity of the restriction specificity of this region of Der p I. The application of this information in the design of peptide-based immunotherapy in the management of allergic responses to HDM is discussed.
AB - The induction of IgE antibodies reactive with the group I allergen of Dermatophagoides species (house dust mite [HDM]), which comprise a major component of the allergic immune response in HDM-atopic individuals, is dependent on the functional activity of specific CD4 + T cells. In this report we demonstrate that for a particular HDM-atopic individual the T-cell response to the group I allergen of Dermatophagoides pteronyssinus (Der p I) is limited to a single region (residues 101-143) of the protein. By mapping the fine antigen specificity with T-cell clones, we observed that the sequence 101-131 of Der p I contains a cluster of at least three overlapping T-cell epitopes. Analysis of the HLA class II restriction specificity of the T-cell clones revealed that the T-cell epitope, residues 110-131, was restricted by HLA-DRB1 *0101. In contrast, peptide Der p 1, 110-119 was recognized in association with HLA-DPB1 *0402. However, the ability of cloned T cells to proliferate to the peptide Der p 1, 107-119 presented by HLA-DPB1 *0401, HLA-DPB1 *0402, and HLA-DPB1 *0501 expressing accessory cells illustrates the heterogeneity of the restriction specificity of this region of Der p I. The application of this information in the design of peptide-based immunotherapy in the management of allergic responses to HDM is discussed.
KW - Der p 1
KW - HLA-DR
KW - house dust mite
KW - Peptides
KW - T-cell epitopes
UR - http://www.scopus.com/inward/record.url?scp=0028360199&partnerID=8YFLogxK
U2 - 10.1016/0091-6749(94)90383-2
DO - 10.1016/0091-6749(94)90383-2
M3 - Article
C2 - 7514196
AN - SCOPUS:0028360199
VL - 93
SP - 891
EP - 899
JO - The Journal of Allergy and Clinical Immunology
JF - The Journal of Allergy and Clinical Immunology
SN - 0091-6749
IS - 5
ER -