Overexpression of alcohol dehydrogenase or pyruvate decarboxylase improves growth of hairy roots at reduced oxygen concentrations

Tien-Li Shiao, Marc H Ellis, Rudy Dolferus, Elizabeth S Dennis, Pauline Mavis Doran

Research output: Contribution to journalArticleResearchpeer-review

47 Citations (Scopus)

Abstract

Overexpression of Arabidopsis thaliana genes for the fermentation enzymes, alcohol dehydrogenase and pyruvate decarboxylase, improved the tolerance of A. thaliana hairy roots to low oxygen conditions. Whereas the specific growth rate of untransformed hairy roots in shake flasks and in a multiple-tube recirculation bioreactor declined significantly with decreasing oxygen tension down to 25 air saturation, growth of the transformant root lines was maintained at rates similar to those achieved with full aeration. This work demonstrates that altering the expression of selected genes involved in anaerobic metabolism can alleviate the problems of oxygen deficiency in hairy root cultures caused by poor mixing and mass transfer conditions.
Original languageEnglish
Pages (from-to)455 - 461
Number of pages7
JournalBiotechnology and Bioengineering
Volume77
Issue number4
DOIs
Publication statusPublished - 2002
Externally publishedYes

Cite this