Over-expression of DMRT1 induces the male pathway in embryonic chicken gonads

Luke S Lambeth, Christopher S. Raymond, Kelly N Roeszler, Asato Kuroiwa, Tomohiro Nakata, David Zarkower, Craig A. Smith

Research output: Contribution to journalArticleResearchpeer-review

47 Citations (Scopus)

Abstract

DMRT1 encodes a conserved transcription factor with an essential role in gonadal function. In the chicken, DMRT1 in located on the Z sex chromosome and is currently the best candidate master regulator of avian gonadal sex differentiation. We previously showed that knockdown of DMRT1 expression during the period of sexual differentiation induces feminisation of male embryonic chicken gonads. This gene is therefore necessary for proper testis development in the chicken. However, whether it is sufficient to induce testicular differentiation has remained unresolved. We show here that over-expression of DMRT1 induces male pathway genes and antagonises the female pathway in embryonic chicken gonads. Ectopic DMRT1 expression in female gonads induces localised SOX9 and AMH expression. It also induces expression of the recently identified Z-linked male factor, Hemogen (HEMGN). Masculinised gonads show evidence of cord-like structures and retarded female-type cortical development. Furthermore, expression of the critical feminising enzyme, aromatase, is reduced in the presence of over-expressed DMRT1. These data indicate that DMRT1 is an essential sex-linked regulator of gonadal differentiation in avians, and that it likely acts via a dosage mechanism established through the lack of global Z dosage compensation in birds.

Original languageEnglish
Pages (from-to)160-172
Number of pages13
JournalDevelopmental Biology
Volume389
Issue number2
DOIs
Publication statusPublished - 15 May 2014
Externally publishedYes

Keywords

  • AMH
  • DMRT1
  • Embryonic chicken gonad
  • HEMGN
  • Sex determination
  • SOX9

Cite this

Lambeth, L. S., Raymond, C. S., Roeszler, K. N., Kuroiwa, A., Nakata, T., Zarkower, D., & Smith, C. A. (2014). Over-expression of DMRT1 induces the male pathway in embryonic chicken gonads. Developmental Biology, 389(2), 160-172. https://doi.org/10.1016/j.ydbio.2014.02.012
Lambeth, Luke S ; Raymond, Christopher S. ; Roeszler, Kelly N ; Kuroiwa, Asato ; Nakata, Tomohiro ; Zarkower, David ; Smith, Craig A. / Over-expression of DMRT1 induces the male pathway in embryonic chicken gonads. In: Developmental Biology. 2014 ; Vol. 389, No. 2. pp. 160-172.
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Lambeth, LS, Raymond, CS, Roeszler, KN, Kuroiwa, A, Nakata, T, Zarkower, D & Smith, CA 2014, 'Over-expression of DMRT1 induces the male pathway in embryonic chicken gonads', Developmental Biology, vol. 389, no. 2, pp. 160-172. https://doi.org/10.1016/j.ydbio.2014.02.012

Over-expression of DMRT1 induces the male pathway in embryonic chicken gonads. / Lambeth, Luke S; Raymond, Christopher S.; Roeszler, Kelly N; Kuroiwa, Asato; Nakata, Tomohiro; Zarkower, David; Smith, Craig A.

In: Developmental Biology, Vol. 389, No. 2, 15.05.2014, p. 160-172.

Research output: Contribution to journalArticleResearchpeer-review

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Lambeth LS, Raymond CS, Roeszler KN, Kuroiwa A, Nakata T, Zarkower D et al. Over-expression of DMRT1 induces the male pathway in embryonic chicken gonads. Developmental Biology. 2014 May 15;389(2):160-172. https://doi.org/10.1016/j.ydbio.2014.02.012