Organosilica particles for DNA screening applications

Simon Corrie, Gwen Lawrie, Bronwyn Battersby, Matt Trau

Research output: Chapter in Book/Report/Conference proceedingConference PaperOther

Abstract

Particle-based libraries for chemical screening are of interest for robust biochemical assays and molecular diagnostic applications. Silica particles are chemically stable and possess two separate chemical modification pathways that facilitate the attachment of fluorescent dyes for encoding purposes and specific oligonucleotides for hybridization assays. Characterization of surface modifications of the particles are presented and quantitation of oligonucleotide probes on the particle surface is described. The application of the particles in biological screening experiments is presented by means of a single base mismatch detection assay.

Original languageEnglish
Title of host publicationProceedings of the 2006 International Conference on Nanoscience and Nanotechnology, ICONN
Pages250-253
Number of pages4
DOIs
Publication statusPublished - 1 Dec 2006
Externally publishedYes
Event2006 International Conference on Nanoscience and Nanotechnology, ICONN 2006 - Brisbane, Australia
Duration: 3 Jul 20066 Jul 2006

Publication series

NameProceedings of the 2006 International Conference on Nanoscience and Nanotechnology, ICONN

Conference

Conference2006 International Conference on Nanoscience and Nanotechnology, ICONN 2006
CountryAustralia
CityBrisbane
Period3/07/066/07/06

Keywords

  • 3-Mercaptopropyl trimethoxysilane
  • DNA hybridization
  • Flow cytometry
  • Particles
  • Surface modifications

Cite this

Corrie, S., Lawrie, G., Battersby, B., & Trau, M. (2006). Organosilica particles for DNA screening applications. In Proceedings of the 2006 International Conference on Nanoscience and Nanotechnology, ICONN (pp. 250-253). [4143379] (Proceedings of the 2006 International Conference on Nanoscience and Nanotechnology, ICONN). https://doi.org/10.1109/ICONN.2006.340599