Abstract
Particle-based libraries for chemical screening are of interest for robust biochemical assays and molecular diagnostic applications. Silica particles are chemically stable and possess two separate chemical modification pathways that facilitate the attachment of fluorescent dyes for encoding purposes and specific oligonucleotides for hybridization assays. Characterization of surface modifications of the particles are presented and quantitation of oligonucleotide probes on the particle surface is described. The application of the particles in biological screening experiments is presented by means of a single base mismatch detection assay.
Original language | English |
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Title of host publication | Proceedings of the 2006 International Conference on Nanoscience and Nanotechnology, ICONN |
Pages | 250-253 |
Number of pages | 4 |
DOIs | |
Publication status | Published - 1 Dec 2006 |
Externally published | Yes |
Event | International Conference on Nanoscience and Nanotechnology (ICONN) 2006 - Brisbane, Australia Duration: 3 Jul 2006 → 6 Jul 2006 Conference number: 1st |
Conference
Conference | International Conference on Nanoscience and Nanotechnology (ICONN) 2006 |
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Abbreviated title | ICONN 2006 |
Country/Territory | Australia |
City | Brisbane |
Period | 3/07/06 → 6/07/06 |
Keywords
- 3-Mercaptopropyl trimethoxysilane
- DNA hybridization
- Flow cytometry
- Particles
- Surface modifications