Organization of ribosomal RNA genes from the footrot pathogen Dichelobacter nodosus

Sharon La Fontaine, Julian I. Rood

Research output: Contribution to journalArticleResearchpeer-review

13 Citations (Scopus)

Abstract

Southern hybridization analysis revealed that there were three rrn loci within the genome of Dichelobacter nodosus, the causative organism of ovine footrot. These loci (rrA, rrB and rrC) were isolated on recombinant lambda clones, and comprised 16S, 23S and 5S rRNA genes closely linked in that order. Sequence and primer extension analysis revealed the presence of putative genes encoding tRNA(Ile) and tRNA(Ala) within the 16S-23S spacer region, as well as a number of potential regulatory features. These elements included a single promoter, which was mapped upstream of the 16S rRNA gene and which was similar to Escherichia coil consensus promoter sequences, an AT-rich upstream region, a GC-rich motif that may be involved in stringent control, leader and spacer antitermination sequences, sites for ribonuclease processing, and a putative factor-independent terminator sequence. Potential open reading frames (ORFs) were identified within the regions flanking the rrn loci, with identical copies of the 3' terminal ORF present downstream of each rRNA operon. Determination of the complete sequence of the 5S rRNA gene, and derivation of the 5S rRNA secondary structure, further substantiated the 16S rRNA-based placement of D. nodosus within the gamma division of the Proteobacteria.

Original languageEnglish
Pages (from-to)889-899
Number of pages11
JournalMicrobiology
Volume142
Issue number4
DOIs
Publication statusPublished - 1 Jan 1996

Keywords

  • Dichelobacter nodosus
  • Footrot
  • Phylogeny
  • rrn operon
  • rRNA genes

Cite this

@article{dc23a201e38f4dc7a831383503559b9a,
title = "Organization of ribosomal RNA genes from the footrot pathogen Dichelobacter nodosus",
abstract = "Southern hybridization analysis revealed that there were three rrn loci within the genome of Dichelobacter nodosus, the causative organism of ovine footrot. These loci (rrA, rrB and rrC) were isolated on recombinant lambda clones, and comprised 16S, 23S and 5S rRNA genes closely linked in that order. Sequence and primer extension analysis revealed the presence of putative genes encoding tRNA(Ile) and tRNA(Ala) within the 16S-23S spacer region, as well as a number of potential regulatory features. These elements included a single promoter, which was mapped upstream of the 16S rRNA gene and which was similar to Escherichia coil consensus promoter sequences, an AT-rich upstream region, a GC-rich motif that may be involved in stringent control, leader and spacer antitermination sequences, sites for ribonuclease processing, and a putative factor-independent terminator sequence. Potential open reading frames (ORFs) were identified within the regions flanking the rrn loci, with identical copies of the 3' terminal ORF present downstream of each rRNA operon. Determination of the complete sequence of the 5S rRNA gene, and derivation of the 5S rRNA secondary structure, further substantiated the 16S rRNA-based placement of D. nodosus within the gamma division of the Proteobacteria.",
keywords = "Dichelobacter nodosus, Footrot, Phylogeny, rrn operon, rRNA genes",
author = "{La Fontaine}, Sharon and Rood, {Julian I.}",
year = "1996",
month = "1",
day = "1",
doi = "10.1099/00221287-142-4-889",
language = "English",
volume = "142",
pages = "889--899",
journal = "Microbiology-Sgm",
issn = "1350-0872",
number = "4",

}

Organization of ribosomal RNA genes from the footrot pathogen Dichelobacter nodosus. / La Fontaine, Sharon; Rood, Julian I.

In: Microbiology, Vol. 142, No. 4, 01.01.1996, p. 889-899.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Organization of ribosomal RNA genes from the footrot pathogen Dichelobacter nodosus

AU - La Fontaine, Sharon

AU - Rood, Julian I.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - Southern hybridization analysis revealed that there were three rrn loci within the genome of Dichelobacter nodosus, the causative organism of ovine footrot. These loci (rrA, rrB and rrC) were isolated on recombinant lambda clones, and comprised 16S, 23S and 5S rRNA genes closely linked in that order. Sequence and primer extension analysis revealed the presence of putative genes encoding tRNA(Ile) and tRNA(Ala) within the 16S-23S spacer region, as well as a number of potential regulatory features. These elements included a single promoter, which was mapped upstream of the 16S rRNA gene and which was similar to Escherichia coil consensus promoter sequences, an AT-rich upstream region, a GC-rich motif that may be involved in stringent control, leader and spacer antitermination sequences, sites for ribonuclease processing, and a putative factor-independent terminator sequence. Potential open reading frames (ORFs) were identified within the regions flanking the rrn loci, with identical copies of the 3' terminal ORF present downstream of each rRNA operon. Determination of the complete sequence of the 5S rRNA gene, and derivation of the 5S rRNA secondary structure, further substantiated the 16S rRNA-based placement of D. nodosus within the gamma division of the Proteobacteria.

AB - Southern hybridization analysis revealed that there were three rrn loci within the genome of Dichelobacter nodosus, the causative organism of ovine footrot. These loci (rrA, rrB and rrC) were isolated on recombinant lambda clones, and comprised 16S, 23S and 5S rRNA genes closely linked in that order. Sequence and primer extension analysis revealed the presence of putative genes encoding tRNA(Ile) and tRNA(Ala) within the 16S-23S spacer region, as well as a number of potential regulatory features. These elements included a single promoter, which was mapped upstream of the 16S rRNA gene and which was similar to Escherichia coil consensus promoter sequences, an AT-rich upstream region, a GC-rich motif that may be involved in stringent control, leader and spacer antitermination sequences, sites for ribonuclease processing, and a putative factor-independent terminator sequence. Potential open reading frames (ORFs) were identified within the regions flanking the rrn loci, with identical copies of the 3' terminal ORF present downstream of each rRNA operon. Determination of the complete sequence of the 5S rRNA gene, and derivation of the 5S rRNA secondary structure, further substantiated the 16S rRNA-based placement of D. nodosus within the gamma division of the Proteobacteria.

KW - Dichelobacter nodosus

KW - Footrot

KW - Phylogeny

KW - rrn operon

KW - rRNA genes

UR - http://www.scopus.com/inward/record.url?scp=0029876169&partnerID=8YFLogxK

U2 - 10.1099/00221287-142-4-889

DO - 10.1099/00221287-142-4-889

M3 - Article

C2 - 8936315

AN - SCOPUS:0029876169

VL - 142

SP - 889

EP - 899

JO - Microbiology-Sgm

JF - Microbiology-Sgm

SN - 1350-0872

IS - 4

ER -