Ordered Silicon Pillar Arrays Prepared by Electrochemical Micromachining: Substrates for High-Efficiency Cell Transfection

Frances J. Harding; Salvatore Surdo; Bahman Delalat; Chiara Cozzi; Roey Elnathan; Stan Gronthos; Nicolas H. Voelcker; Giuseppe Barillaro

doi:10.1021/acsami.6b07850

Ordered Silicon Pillar Arrays Prepared by Electrochemical Micromachining: Substrates for High-Efficiency Cell Transfection

Frances J. Harding, Salvatore Surdo, Bahman Delalat, Chiara Cozzi, Roey Elnathan, Stan Gronthos, Nicolas H. Voelcker, Giuseppe Barillaro

Research output: Contribution to journal › Article › Research › peer-review

47 Citations (Scopus)

Abstract

Ordered arrays of silicon nano- to microscale pillars are used to enable biomolecular trafficking into primary human cells, consistently demonstrating high transfection efficiency can be achieved with broader and taller pillars than reported to date. Cell morphology on the pillar arrays is often strikingly elongated. Investigation of the cellular interaction with the pillar reveals that cells are suspended on pillar tips and do not interact with the substrate between the pillars. Although cells remain suspended on pillar tips, acute local deformation of the cell membrane was noted, allowing pillar tips to penetrate the cell interior, while retaining cell viability.

Original language	English
Pages (from-to)	29197-29202
Number of pages	6
Journal	ACS Applied Materials & Interfaces
Volume	8
Issue number	43
DOIs	https://doi.org/10.1021/acsami.6b07850
Publication status	Published - 2 Nov 2016
Externally published	Yes

Keywords

cell alignment
pillars
silicon nanostructures
topography
transfection

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10.1021/acsami.6b07850

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abstract = "Ordered arrays of silicon nano- to microscale pillars are used to enable biomolecular trafficking into primary human cells, consistently demonstrating high transfection efficiency can be achieved with broader and taller pillars than reported to date. Cell morphology on the pillar arrays is often strikingly elongated. Investigation of the cellular interaction with the pillar reveals that cells are suspended on pillar tips and do not interact with the substrate between the pillars. Although cells remain suspended on pillar tips, acute local deformation of the cell membrane was noted, allowing pillar tips to penetrate the cell interior, while retaining cell viability.",

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AU - Surdo, Salvatore

AU - Delalat, Bahman

AU - Cozzi, Chiara

AU - Elnathan, Roey

AU - Gronthos, Stan

AU - Voelcker, Nicolas H.

AU - Barillaro, Giuseppe

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AB - Ordered arrays of silicon nano- to microscale pillars are used to enable biomolecular trafficking into primary human cells, consistently demonstrating high transfection efficiency can be achieved with broader and taller pillars than reported to date. Cell morphology on the pillar arrays is often strikingly elongated. Investigation of the cellular interaction with the pillar reveals that cells are suspended on pillar tips and do not interact with the substrate between the pillars. Although cells remain suspended on pillar tips, acute local deformation of the cell membrane was noted, allowing pillar tips to penetrate the cell interior, while retaining cell viability.

KW - cell alignment

KW - pillars

KW - silicon nanostructures

KW - topography

KW - transfection

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Ordered Silicon Pillar Arrays Prepared by Electrochemical Micromachining: Substrates for High-Efficiency Cell Transfection

Abstract

Keywords

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