TY - JOUR
T1 - Ordered mesoporous alumina with ultra-large pores as an efficient absorbent for selective bioenrichment
AU - Wei, Jing
AU - Ren, Yuan
AU - Luo, Wei
AU - Sun, Zhenkun
AU - Cheng, Xiaowei
AU - Li, Yuhui
AU - Deng, Yonghui
AU - Elzatahry, Ahmed A.
AU - Al-Dahyan, Daifallah
AU - Zhao, Dongyuan
PY - 2017/3/14
Y1 - 2017/3/14
N2 - Alumina has recently turned out to be effective in enrichment of biomacrolecules like phosphopeptides due to its good affinity to phosphor groups. Ordered mesoporous alumina (OMA) materials with high surface areas, regular porous structures, and large pore size are an ideal absorbent for the enrichment of phosphopeptides. Herein, a ligand-assisted solvent evaporation induced coassembly route is developed to synthesize OMA materials with an ultralarge pore size (16.0-18.9 nm) using a high-molecular-weight poly(ethylene oxide)-b-polystyrene (PEO-b-PS) as a soft template, aluminum acetylacetonate as a precursor, and tetrahydrofuran as a solvent. The obtained ordered mesoporous alumina shows high surface area (114-197 m2/g), large pore volume (0.16-0.34 cm3/g), and high thermal stability (up to 900 °C). The OMA materials show crystalline γ-Al2O3 frameworks with crystal size of ∼11 nm after calcination at 900 °C in air. Because of their high surface area, ultralarge pore size, and rich Lewis acid sites, the obtained OMA materials are demonstrated to be an excellent bioabsorbent in enriching phosphopeptides selectively from protein digestions with ultralow concentrations (2 × 10-9 M), even from more complex samples from human serum.
AB - Alumina has recently turned out to be effective in enrichment of biomacrolecules like phosphopeptides due to its good affinity to phosphor groups. Ordered mesoporous alumina (OMA) materials with high surface areas, regular porous structures, and large pore size are an ideal absorbent for the enrichment of phosphopeptides. Herein, a ligand-assisted solvent evaporation induced coassembly route is developed to synthesize OMA materials with an ultralarge pore size (16.0-18.9 nm) using a high-molecular-weight poly(ethylene oxide)-b-polystyrene (PEO-b-PS) as a soft template, aluminum acetylacetonate as a precursor, and tetrahydrofuran as a solvent. The obtained ordered mesoporous alumina shows high surface area (114-197 m2/g), large pore volume (0.16-0.34 cm3/g), and high thermal stability (up to 900 °C). The OMA materials show crystalline γ-Al2O3 frameworks with crystal size of ∼11 nm after calcination at 900 °C in air. Because of their high surface area, ultralarge pore size, and rich Lewis acid sites, the obtained OMA materials are demonstrated to be an excellent bioabsorbent in enriching phosphopeptides selectively from protein digestions with ultralow concentrations (2 × 10-9 M), even from more complex samples from human serum.
UR - http://www.scopus.com/inward/record.url?scp=85015776465&partnerID=8YFLogxK
U2 - 10.1021/acs.chemmater.6b05032
DO - 10.1021/acs.chemmater.6b05032
M3 - Article
AN - SCOPUS:85015776465
VL - 29
SP - 2211
EP - 2217
JO - Chemistry of Materials
JF - Chemistry of Materials
SN - 0897-4756
IS - 5
ER -