Oral vaccination of mice against tetanus by use of a live attenuated Salmonella carrier

N. F. Fairweather; S. N. Chatfield; A. J. Makoff; R. A. Strugnell; J. Bester; D. J. Maskell; G. Dougan

Oral vaccination of mice against tetanus by use of a live attenuated Salmonella carrier

N. F. Fairweather, S. N. Chatfield, A. J. Makoff, R. A. Strugnell, J. Bester, D. J. Maskell, G. Dougan

Research output: Contribution to journal › Article › Research › peer-review

Abstract

A Salmonella typhimurium aroA mutant has been used as a live carrier to immunize mice against tetanus. Plasmid pTETtac4, which expresses a 50-kilodalton fragment of tetanus toxin (fragment C) under the control of the tac promoter, was introduced into SL3261 aroA. When used as a live vaccine and administered orally or intravenously, this strain was able to induce protective immunity in mice against a lethal tetanus toxin challenge. When plasmid pTETtac2, which contains the lacI gene, was used, no immunity was obtained, indicating that the expression of fragment C was repressed in vivo. We believe that this is the first example of a successful oral vaccination that uses an attenuated bacterial carrier to deliver a protective antigen derived from tetanus toxin.

Original language	English
Pages (from-to)	1323-1326
Number of pages	4
Journal	Infection and Immunity
Volume	58
Issue number	5
Publication status	Published - 1 Jan 1990
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

@article{03128d74c712404aa9fe2836e3b3a0d9,

title = "Oral vaccination of mice against tetanus by use of a live attenuated Salmonella carrier",

abstract = "A Salmonella typhimurium aroA mutant has been used as a live carrier to immunize mice against tetanus. Plasmid pTETtac4, which expresses a 50-kilodalton fragment of tetanus toxin (fragment C) under the control of the tac promoter, was introduced into SL3261 aroA. When used as a live vaccine and administered orally or intravenously, this strain was able to induce protective immunity in mice against a lethal tetanus toxin challenge. When plasmid pTETtac2, which contains the lacI gene, was used, no immunity was obtained, indicating that the expression of fragment C was repressed in vivo. We believe that this is the first example of a successful oral vaccination that uses an attenuated bacterial carrier to deliver a protective antigen derived from tetanus toxin.",

author = "Fairweather, {N. F.} and Chatfield, {S. N.} and Makoff, {A. J.} and Strugnell, {R. A.} and J. Bester and Maskell, {D. J.} and G. Dougan",

year = "1990",

month = jan,

day = "1",

language = "English",

volume = "58",

pages = "1323--1326",

journal = "Infection and Immunity",

issn = "1098-5522",

publisher = "American Society for Microbiology",

number = "5",

}

TY - JOUR

T1 - Oral vaccination of mice against tetanus by use of a live attenuated Salmonella carrier

AU - Fairweather, N. F.

AU - Chatfield, S. N.

AU - Makoff, A. J.

AU - Strugnell, R. A.

AU - Bester, J.

AU - Maskell, D. J.

AU - Dougan, G.

PY - 1990/1/1

Y1 - 1990/1/1

N2 - A Salmonella typhimurium aroA mutant has been used as a live carrier to immunize mice against tetanus. Plasmid pTETtac4, which expresses a 50-kilodalton fragment of tetanus toxin (fragment C) under the control of the tac promoter, was introduced into SL3261 aroA. When used as a live vaccine and administered orally or intravenously, this strain was able to induce protective immunity in mice against a lethal tetanus toxin challenge. When plasmid pTETtac2, which contains the lacI gene, was used, no immunity was obtained, indicating that the expression of fragment C was repressed in vivo. We believe that this is the first example of a successful oral vaccination that uses an attenuated bacterial carrier to deliver a protective antigen derived from tetanus toxin.

AB - A Salmonella typhimurium aroA mutant has been used as a live carrier to immunize mice against tetanus. Plasmid pTETtac4, which expresses a 50-kilodalton fragment of tetanus toxin (fragment C) under the control of the tac promoter, was introduced into SL3261 aroA. When used as a live vaccine and administered orally or intravenously, this strain was able to induce protective immunity in mice against a lethal tetanus toxin challenge. When plasmid pTETtac2, which contains the lacI gene, was used, no immunity was obtained, indicating that the expression of fragment C was repressed in vivo. We believe that this is the first example of a successful oral vaccination that uses an attenuated bacterial carrier to deliver a protective antigen derived from tetanus toxin.

UR - http://www.scopus.com/inward/record.url?scp=0025269567&partnerID=8YFLogxK

M3 - Article

C2 - 2182542

AN - SCOPUS:0025269567

SN - 1098-5522

VL - 58

SP - 1323

EP - 1326

JO - Infection and Immunity

JF - Infection and Immunity

IS - 5

ER -

Oral vaccination of mice against tetanus by use of a live attenuated Salmonella carrier

Abstract

UN SDGs

Other files and links

Cite this