Optimization of the expression of recombinant human activin A in the yeast Pichia pastoris

Dale Fredericks, Robert Clay, Tracy Warner, Anne O'Connor, David de Kretser, Milton Thomas William Hearn

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)

Abstract

We report a new procedure to express recombinant human activin A using the methanolic yeast, Pichia pastoris. Optimization of culture procedures has involved comprehensive examination of the effects of culture vessel shape, volume of broth in the induction and expression cultures, methanol concentration, culturing temperature, and pH of the expression cultures. After this optimization, as well as modification of the native cleavage sites, a laboratory scale procedure has been established which routinely produced 2-10 mg/L amounts of this vital growth factor in the highly efficient, eukaryotic yeast system. This system avoids the need to produce this protein and similar TGF-beta proteins in mammalian cell lines which, in addition to being costly, produce many native binding partners of these cystine knot proteins, a factor which can dramatically affect yields of the target protein.
Original languageEnglish
Pages (from-to)372 - 383
Number of pages12
JournalBiotechnology Progress
Volume26
Issue number2
DOIs
Publication statusPublished - 2010

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