Optimization of Newcastle disease virus production in T-flask

Mohammad Azmir Arifin, Maizirwan Mel, Siti Hajar Salim, Mohd Ismail Abdul Karim, Sharifah Syed Hassan

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    Abstract

    In the present study, the production of lentogenic Asplin F strain of Newcastle disease virus by using cell culture method was studied. Experiments were carried out in T-flasks to investigate the effects of serum concentration in the culture medium during virus replication phase and multiplicity of infection (MOI) on ND virus propagation in DF-1 cells. Results show that virus infectivity titre of 6.62 x 107 TCID50/ml was achieved when serum concentration of 0.5 and MOI of 20 was used
    Original languageEnglish
    Pages (from-to)18816 - 18823
    Number of pages8
    JournalAfrican Journal of Biotechnology
    Volume10
    Issue number81
    DOIs
    Publication statusPublished - 2011

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