Abstract
In the present study, the production of lentogenic Asplin F strain of Newcastle disease virus by
using cell culture method was studied. Experiments were carried out in T-flasks to investigate the
effects of serum concentration in the culture medium during virus replication phase and multiplicity of
infection (MOI) on ND virus propagation in DF-1 cells. Results show that virus infectivity titre of 6.62 x
107 TCID50/ml was achieved when serum concentration of 0.5 and MOI of 20 was used
Original language | English |
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Pages (from-to) | 18816 - 18823 |
Number of pages | 8 |
Journal | African Journal of Biotechnology |
Volume | 10 |
Issue number | 81 |
DOIs | |
Publication status | Published - 2011 |