Optimization of a native gel electrophoretic process for the purification of intracellular green fluorescent protein from intact Escherichia coli cells

Few Ne Chew, Wen Siang Tan, Tau Chuan Ling, Beng Ti Tey

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1 Citation (Scopus)


Intracellular green fluorescent protein (GFP) can be separated and purified from intact Escherichia coli cells by a preparative native polyacrylamide gel electrophoresis (n-PAGE). The effects of operating parameters such as the volume and concentration of feedstock and the pore size and height of resolving gel on the purity and yield of GFP were studied using a 1.7 cm internal diameter gel column. The optimum conditions for this preparative n-PAGE operation were determined to be 100 μl of 15% (w/v) feedstock and a 12% (w/v) resolving gel with a gel height of 2 cm. The purity and yield of the recovered GFP were 98 and 88%, respectively. The results of scalability studies show that the ratio of feedstock volume to cross-sectional area of the column is an important consideration for scaling up the preparative n-PAGE.

Original languageEnglish
Pages (from-to)399-403
Number of pages5
JournalProcess Biochemistry
Issue number1
Publication statusPublished - Jan 2011
Externally publishedYes


  • Escherichia coli
  • Green fluorescent protein
  • Optimization
  • Preparative native polyacrylamide gel electrophoresis
  • Protein purification
  • Scale-up criterion

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