Optimisation of Fluorescence Detection for Polyaromatic Hydrocarbon Determination by Using High Performance Liquid Chromatography

P. J. Marriott, P. D. Carpenter, P. H. Brady, M. J. McCormick, A. J. Griffiths, T. S.G. Hatvani, S. G. Rasdell

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18 Citations (Scopus)

Abstract

The procedures needed for the optimisation of programmable fluorescence detectors have been investigated. We found that wavelength programs had to be developed from data obtained from the particular detector for which the program was to be used - programs reported in the literature or developed from data obtained on other instruments gave substantially lower sensitivities. The wavelength program we developed was a compromise between: chromatographic separation, abundance and relative sensitivity of each PAH; the ease with which a timed event could be included in the elution profile and; the relative importance of each PAH to the analyst. The success of programmable fluorescence detection was dependant on the reproducibility with which the detector was able to reset specified wavelengths, with corresponding detector response variability of up to 20% for one new detector.

Original languageEnglish
Pages (from-to)3229-3247
Number of pages19
JournalJournal of Liquid Chromatography
Volume16
Issue number15
DOIs
Publication statusPublished - 1 Jan 1993
Externally publishedYes

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