TY - JOUR
T1 - Optimisation of enzymatic directed interesterification of palm oil for high yield of triunsaturated triacylglycerol using response surface methodology
AU - Dian, Noor Lida Habi Mat
AU - Sahri, Miskandar Mat
AU - Ping, Tan Chin
AU - Ying, Lee Yee
AU - Ming, Lai Oi
N1 - Funding Information:
The authors acknowledge MPOB for the financial support to this research and permission to publish this article. The authors also acknowledge Isham Ismail and Muhamad Adrina Malek of Oils and Fats Technology Centre (OFTEC), MPOB for their contribution in ensuring the success of this study.
Publisher Copyright:
© 2018 Lembaga Minyak Sawit Malaysia. All rights reserved.
PY - 2018/12
Y1 - 2018/12
N2 - Unsaturated fatty acids (USAFA), especially monounsaturated fatty acids (MUFA), has been shown to provide substantial health benefits. This study attempted to increase the triunsaturated (U3) triacylglycerols (TAG) content in palm oil (PO) via lipase-catalysed directed interesterification (EDIE), to enable the production of a liquid fraction that is high in USAFA, especially MUFA. A response surface methodology with three factors and five levels in a central composite design was employed for optimisation of the EDIE conditions for the U3 TAG production. The effects of reaction temperature (20°C-50°C), reaction time (12-28 hr) and enzyme load (2%-18%) on the U3 TAG and by-products yields were investigated. Well-fitted quadratic and linear models were successfully established for both responses. All processing conditions affected significantly (p<0.05) U3 TAG yield. By-products yield was affected by reaction time and temperature. Although increasing reaction temperature and enzyme load, and extending the reaction time resulted in a higher U3 TAG yield, a higher amount of by-products were formed as well. The optimised processing conditions were a reaction temperature of 30°C and reaction time of 18 hr, when immobilised lipase enzyme load was set at 10% (w/w), and resulted in yields of about 27% of U3 TAG and 23% of by-products.
AB - Unsaturated fatty acids (USAFA), especially monounsaturated fatty acids (MUFA), has been shown to provide substantial health benefits. This study attempted to increase the triunsaturated (U3) triacylglycerols (TAG) content in palm oil (PO) via lipase-catalysed directed interesterification (EDIE), to enable the production of a liquid fraction that is high in USAFA, especially MUFA. A response surface methodology with three factors and five levels in a central composite design was employed for optimisation of the EDIE conditions for the U3 TAG production. The effects of reaction temperature (20°C-50°C), reaction time (12-28 hr) and enzyme load (2%-18%) on the U3 TAG and by-products yields were investigated. Well-fitted quadratic and linear models were successfully established for both responses. All processing conditions affected significantly (p<0.05) U3 TAG yield. By-products yield was affected by reaction time and temperature. Although increasing reaction temperature and enzyme load, and extending the reaction time resulted in a higher U3 TAG yield, a higher amount of by-products were formed as well. The optimised processing conditions were a reaction temperature of 30°C and reaction time of 18 hr, when immobilised lipase enzyme load was set at 10% (w/w), and resulted in yields of about 27% of U3 TAG and 23% of by-products.
KW - Enzymatic directed interesterification
KW - Palm oil
KW - Response surface methodology
KW - Triunsaturated triacylglycerol
KW - Unsaturated fatty acid
UR - http://www.scopus.com/inward/record.url?scp=85060738218&partnerID=8YFLogxK
U2 - 10.21894/jopr.2018.0050
DO - 10.21894/jopr.2018.0050
M3 - Article
AN - SCOPUS:85060738218
SN - 1511-2780
VL - 30
SP - 602
EP - 616
JO - Journal of Oil Palm Research
JF - Journal of Oil Palm Research
IS - 4
ER -