Optimal assay design for determining the in vitro sensitivity of ring stage Plasmodium falciparum to artemisinins

Stanley C. Xie; Con Dogovski; Shannon Kenny; Leann Tilley; Nectarios Klonis

doi:10.1016/j.ijpara.2014.07.008

Optimal assay design for determining the in vitro sensitivity of ring stage Plasmodium falciparum to artemisinins

Stanley C. Xie
, Con Dogovski
, Shannon Kenny
, Leann Tilley
, Nectarios Klonis

Research output: Contribution to journal › Review Article › Research › peer-review

22 Citations (Scopus)

Abstract

Recent reports demonstrate that failure of artemisinin-based antimalarial therapies is associated with an altered response of early blood stage Plasmodium falciparum. This has led to increased interest in the use of pulse assays that mimic clinical drug exposure for analysing artemisinin sensitivity of highly synchronised ring stage parasites. We report a methodology for the reliable execution of drug pulse assays and detail a synchronisation strategy that produces well-defined tightly synchronised ring stage cultures in a convenient time-frame.

Original language	English
Pages (from-to)	893-899
Number of pages	7
Journal	International Journal for Parasitology
Volume	44
Issue number	12
DOIs	https://doi.org/10.1016/j.ijpara.2014.07.008
Publication status	Published - 15 Oct 2014
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Artemisinin
Malaria
Plasmodium falciparum
Pulse drug assay
Synchronisation

Access to Document

10.1016/j.ijpara.2014.07.008

Cite this

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title = "Optimal assay design for determining the in vitro sensitivity of ring stage Plasmodium falciparum to artemisinins",

abstract = "Recent reports demonstrate that failure of artemisinin-based antimalarial therapies is associated with an altered response of early blood stage Plasmodium falciparum. This has led to increased interest in the use of pulse assays that mimic clinical drug exposure for analysing artemisinin sensitivity of highly synchronised ring stage parasites. We report a methodology for the reliable execution of drug pulse assays and detail a synchronisation strategy that produces well-defined tightly synchronised ring stage cultures in a convenient time-frame.",

keywords = "Artemisinin, Malaria, Plasmodium falciparum, Pulse drug assay, Synchronisation",

author = "Xie, \{Stanley C.\} and Con Dogovski and Shannon Kenny and Leann Tilley and Nectarios Klonis",

note = "Funding Information: This work is supported by grants from the Australian Research Council and the Australian National Health \& Medical Research Council. We thank Dr Eric Hanssen, Melbourne Advanced Microscopy Facility, Bio21 Institute, University of Melbourne, Australia, for generating the electron tomographic reconstruction of a P. falciparum-infected RBC included on the front cover of this Special Issue. Publisher Copyright: {\textcopyright} 2014.",

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doi = "10.1016/j.ijpara.2014.07.008",

language = "English",

volume = "44",

pages = "893--899",

journal = "International Journal for Parasitology",

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TY - JOUR

T1 - Optimal assay design for determining the in vitro sensitivity of ring stage Plasmodium falciparum to artemisinins

AU - Xie, Stanley C.

AU - Dogovski, Con

AU - Kenny, Shannon

AU - Tilley, Leann

AU - Klonis, Nectarios

N1 - Funding Information: This work is supported by grants from the Australian Research Council and the Australian National Health & Medical Research Council. We thank Dr Eric Hanssen, Melbourne Advanced Microscopy Facility, Bio21 Institute, University of Melbourne, Australia, for generating the electron tomographic reconstruction of a P. falciparum-infected RBC included on the front cover of this Special Issue. Publisher Copyright: © 2014.

PY - 2014/10/15

Y1 - 2014/10/15

N2 - Recent reports demonstrate that failure of artemisinin-based antimalarial therapies is associated with an altered response of early blood stage Plasmodium falciparum. This has led to increased interest in the use of pulse assays that mimic clinical drug exposure for analysing artemisinin sensitivity of highly synchronised ring stage parasites. We report a methodology for the reliable execution of drug pulse assays and detail a synchronisation strategy that produces well-defined tightly synchronised ring stage cultures in a convenient time-frame.

AB - Recent reports demonstrate that failure of artemisinin-based antimalarial therapies is associated with an altered response of early blood stage Plasmodium falciparum. This has led to increased interest in the use of pulse assays that mimic clinical drug exposure for analysing artemisinin sensitivity of highly synchronised ring stage parasites. We report a methodology for the reliable execution of drug pulse assays and detail a synchronisation strategy that produces well-defined tightly synchronised ring stage cultures in a convenient time-frame.

KW - Artemisinin

KW - Malaria

KW - Plasmodium falciparum

KW - Pulse drug assay

KW - Synchronisation

UR - https://www.scopus.com/pages/publications/84908160941

U2 - 10.1016/j.ijpara.2014.07.008

DO - 10.1016/j.ijpara.2014.07.008

M3 - Review Article

C2 - 25161101

AN - SCOPUS:84908160941

SN - 0020-7519

VL - 44

SP - 893

EP - 899

JO - International Journal for Parasitology

JF - International Journal for Parasitology

IS - 12

ER -

Optimal assay design for determining the in vitro sensitivity of ring stage Plasmodium falciparum to artemisinins

Abstract

UN SDGs

Keywords

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