Optical projection tomography as a novel method to visualize and quantitate whole-brain patterns of cell proliferation in the adult zebrafish brain

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Abstract

How distinct cell populations are distributed in three-dimensional space under homeostasis or following injury, neurodegeneration, or with senescence can teach us much about brain-wide patterns and signaling along the neuroaxis. Visualizing individual cell populations in the mature vertebrate central nervous system (CNS) has remained a challenge as a result of difficulty clearing adult brain tissue or limitations in imaging depth or resolution. We have developed a simple clearing and imaging pipeline optimally suited for the adult zebrafish brain to investigate changes in patterns of cell proliferation in wild-type and transgenic backgrounds that can easily be quantified and represented using Fiji and IMARIS software.

Original languageEnglish
Pages (from-to)574-577
Number of pages4
JournalZebrafish
Volume14
Issue number6
DOIs
Publication statusPublished - 1 Dec 2017

Keywords

  • brain development
  • clearing
  • neurogenesis
  • regeneration
  • stem cell proliferation
  • three-dimensional imaging

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