Optical projection tomography as a novel method to visualize and quantitate whole-brain patterns of cell proliferation in the adult zebrafish brain

Research output: Contribution to journalArticleResearchpeer-review

Abstract

How distinct cell populations are distributed in three-dimensional space under homeostasis or following injury, neurodegeneration, or with senescence can teach us much about brain-wide patterns and signaling along the neuroaxis. Visualizing individual cell populations in the mature vertebrate central nervous system (CNS) has remained a challenge as a result of difficulty clearing adult brain tissue or limitations in imaging depth or resolution. We have developed a simple clearing and imaging pipeline optimally suited for the adult zebrafish brain to investigate changes in patterns of cell proliferation in wild-type and transgenic backgrounds that can easily be quantified and represented using Fiji and IMARIS software.

Original languageEnglish
Pages (from-to)574-577
Number of pages4
JournalZebrafish
Volume14
Issue number6
DOIs
Publication statusPublished - 1 Dec 2017

Keywords

  • brain development
  • clearing
  • neurogenesis
  • regeneration
  • stem cell proliferation
  • three-dimensional imaging

Cite this

@article{bfdea91366404be6bc7e539793c88c28,
title = "Optical projection tomography as a novel method to visualize and quantitate whole-brain patterns of cell proliferation in the adult zebrafish brain",
abstract = "How distinct cell populations are distributed in three-dimensional space under homeostasis or following injury, neurodegeneration, or with senescence can teach us much about brain-wide patterns and signaling along the neuroaxis. Visualizing individual cell populations in the mature vertebrate central nervous system (CNS) has remained a challenge as a result of difficulty clearing adult brain tissue or limitations in imaging depth or resolution. We have developed a simple clearing and imaging pipeline optimally suited for the adult zebrafish brain to investigate changes in patterns of cell proliferation in wild-type and transgenic backgrounds that can easily be quantified and represented using Fiji and IMARIS software.",
keywords = "brain development, clearing, neurogenesis, regeneration, stem cell proliferation, three-dimensional imaging",
author = "Lindsey, {Benjamin W.} and Jan Kaslin",
year = "2017",
month = "12",
day = "1",
doi = "10.1089/zeb.2017.1418",
language = "English",
volume = "14",
pages = "574--577",
journal = "Zebrafish",
issn = "1545-8547",
publisher = "Mary Ann Liebert Inc",
number = "6",

}

Optical projection tomography as a novel method to visualize and quantitate whole-brain patterns of cell proliferation in the adult zebrafish brain. / Lindsey, Benjamin W.; Kaslin, Jan.

In: Zebrafish, Vol. 14, No. 6, 01.12.2017, p. 574-577.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Optical projection tomography as a novel method to visualize and quantitate whole-brain patterns of cell proliferation in the adult zebrafish brain

AU - Lindsey, Benjamin W.

AU - Kaslin, Jan

PY - 2017/12/1

Y1 - 2017/12/1

N2 - How distinct cell populations are distributed in three-dimensional space under homeostasis or following injury, neurodegeneration, or with senescence can teach us much about brain-wide patterns and signaling along the neuroaxis. Visualizing individual cell populations in the mature vertebrate central nervous system (CNS) has remained a challenge as a result of difficulty clearing adult brain tissue or limitations in imaging depth or resolution. We have developed a simple clearing and imaging pipeline optimally suited for the adult zebrafish brain to investigate changes in patterns of cell proliferation in wild-type and transgenic backgrounds that can easily be quantified and represented using Fiji and IMARIS software.

AB - How distinct cell populations are distributed in three-dimensional space under homeostasis or following injury, neurodegeneration, or with senescence can teach us much about brain-wide patterns and signaling along the neuroaxis. Visualizing individual cell populations in the mature vertebrate central nervous system (CNS) has remained a challenge as a result of difficulty clearing adult brain tissue or limitations in imaging depth or resolution. We have developed a simple clearing and imaging pipeline optimally suited for the adult zebrafish brain to investigate changes in patterns of cell proliferation in wild-type and transgenic backgrounds that can easily be quantified and represented using Fiji and IMARIS software.

KW - brain development

KW - clearing

KW - neurogenesis

KW - regeneration

KW - stem cell proliferation

KW - three-dimensional imaging

UR - http://www.scopus.com/inward/record.url?scp=85036561533&partnerID=8YFLogxK

U2 - 10.1089/zeb.2017.1418

DO - 10.1089/zeb.2017.1418

M3 - Article

VL - 14

SP - 574

EP - 577

JO - Zebrafish

JF - Zebrafish

SN - 1545-8547

IS - 6

ER -