Optical biosensor based on hollow integrated waveguides

Víctor J. Cadarso, César Fernández-Sánchez, Andreu Llobera, Margarita Darder, Carlos Domínguez

Research output: Contribution to journalArticleResearchpeer-review

17 Citations (Scopus)

Abstract

The first absorbance biosensor based on pure silicon hollow integrated waveguides is presented in this work. With the use of horseradish peroxidase (HRP) as a model recognition element, an enzymatic sensor for the measurement of hydrogen peroxide was fabricated, numerically simulated, and experimentally characterized. Waveguides with widths ranging from 50 to 80 μm, having a depth of 50 μm and lengths up to 5 mm were easily fabricated by just one photolithographic step. These were further modified by covalent immobilization of HRP using silanization chemistry. Simulation studies of the proposed approach showed a sensor linear behavior up to 300 μM H2O2 and a sensitivity of 2.7 × 10-3 AU/μM. Experimental results were in good agreement with the simulated ones. A linear behavior between 10 and 300 μM H2O2, a sensitivity of 3 × 10-3 AU/μM, and a signal-to-noise ratio around 20 dB were attained. Also, kinetic studies of the activity of the immobilized enzyme on the silicon waveguide surface gave an apparent Michaelis-Menten constant of 0.44 mM. The simple technology proposed in this work enables the fabrication of cost-effective, easy-to-use, miniaturized biosensor generic platforms, these being envisioned as excellent candidates for the development of lab-on-a-chip systems.

Original languageEnglish
Pages (from-to)3498-3501
Number of pages4
JournalAnalytical Chemistry
Volume80
Issue number9
DOIs
Publication statusPublished - 1 May 2008

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