Opsonization of malaria-infected erythrocytes activates the inflammasome and enhances inflammatory cytokine secretion by human macrophages

Jingling Zhou, Louise E Ludlow, Wina Hasang, Stephen J Rogerson, Anthony Jaworowski

Research output: Contribution to journalArticleResearchpeer-review

27 Citations (Scopus)

Abstract

Phagocytic clearance, cytokine response and intracellular signalling were measured using IFN-?-primed human monocyte-derived macrophages (MDM) incubated with opsonized and unopsonized trophozoite-stage CS2 IE, a chondroitin sulphate-binding malaria strain. Cytokine secretion was measured by bead array or ELISA, mRNA using quantitative PCR, and activation of NF-?B by Western blot and electrophoretic mobility shift assay. Data were analysed using the Mann?Whitney U test or the Wilcoxon signed rank test as appropriate.
Original languageEnglish
Pages (from-to)1 - 13
Number of pages13
JournalMalaria Journal
Volume11
Issue numberArt. ID: 343
DOIs
Publication statusPublished - 2012

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