Oocytes mount a noncanonical DNA damage response involving APC-Cdh1–mediated proteolysis

Goutham Narayanan Subramanian, Jessica Greaney, Zhe Wei, Olivier Becherel, Martin Lavin, Hayden Anthony Homer

Research output: Contribution to journalArticleResearchpeer-review

21 Citations (Scopus)

Abstract

In mitotic cells, DNA damage induces temporary G2 arrest via inhibitory Cdk1 phosphorylation. In contrast, fully grown G2-stage oocytes readily enter M phase immediately following chemical induction of DNA damage in vitro, indicating that the canonical immediate-response G2/M DNA damage response (DDR) may be deficient. Senataxin (Setx) is involved in RNA/DNA processing and maintaining genome integrity. Here we find that mouse oocytes deleted of Setx accumulate DNA damage when exposed to oxidative stress in vitro and during aging in vivo, after which, surprisingly, they undergo G2 arrest. Moreover, fully grown wild-type oocytes undergo G2 arrest after chemotherapy-induced in vitro damage if an overnight delay is imposed following damage induction. Unexpectedly, this slow-evolving DDR is not mediated by inhibitory Cdk1 phosphorylation but by APC-Cdh1–mediated proteolysis of the Cdk1 activator, cyclin B1, secondary to increased Cdc14Bdependent APC-Cdh1 activation and reduced Emi1-dependent inhibition. Thus, oocytes are unable to respond immediately to DNA damage, but instead mount a G2/M DDR that evolves slowly and involves a phosphorylation-independent proteolytic pathway.

Original languageEnglish
Article numbere201907213
Number of pages18
JournalJournal of Cell Biology
Volume219
Issue number4
DOIs
Publication statusPublished - 6 Apr 2020
Externally publishedYes

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