The sensitivity of land surface energy partitioning to near-surface air temperature (Ta) is a critical issue to understand the interaction between land surface and climatic system. Thus, studies with in situ observed data compiled from various climates and ecosystems are required. The relations derived from such empirical analyses are useful for developing accurate estimation methods of energy partitioning. In this study, the effect of Ta on land surface energy partitioning is evaluated by using flux measurement data compiled from a global network of eddy covariance tower sites (FLUXNET). According to the analysis of 25 FLUXNET sites (60 site-years) data, the Bowen ratio is found to have a linear relation with the bulk surface resistance normalized by aerodynamic and climatological resistance parameters in general, of which the slope and intercept are dependent on Ta. Energy partitioning in warmer atmosphere is less sensitive to changes in land surface conditions. In addition, a negative relation is found between Bowen ratio and Ta, and this relation is stronger above less vegetated surface and under low vapor pressure deficit and low received radiative energy condition. The empirical results obtained in this study are expected to be useful in gaining better understanding of alternating surface energy partitioning under increasing Ta.