On the influence of the incident photon energy on the radiation damage in crystalline biological samples

Manfred S. Weiss, Santosh Panjikar, Christoph Mueller-Dieckmann, Paul A. Tucker

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32 Citations (Scopus)

Abstract

Two series of complete and highly redundant data sets were collected at wavelengths of 1.00 and 2.00 Å on a cadmium derivative of porcine pancreatic elastase (PPE). Radiation damage to the sample was evaluated qualitatively by inspecting consecutive difference electron density maps during the course of the experiment. The nature of the radiation damage was found to be identical at both wavelengths and was localized primarily at the four disulfide bridges of PPE, the cadmium site and the two methionine residues. For a quantitative examination of the radiation damage, the decrease in the peak height of the cadmium ion in various electron density maps was exploited. Again, no significant difference in radiation damage between the two wavelengths was observed. This can be rationalized by considering the wavelength dependencies of the number of diffracted photons versus the number of absorbed photons and the energy deposited in the crystal by the latter.

Original languageEnglish
Pages (from-to)304-309
Number of pages6
JournalJournal of Synchrotron Radiation
Volume12
Issue number3
DOIs
Publication statusPublished - May 2005
Externally publishedYes

Keywords

  • Coherent scattering
  • Disulfide bond
  • Long wavelength
  • Photoelectric absorption
  • Radiation damage
  • X-ray crystallography

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