TY - JOUR
T1 - On-chip separation of Lactobacillus bacteria from yeasts using dielectrophoresis
AU - Khoshmanesh, Khashayar
AU - Baratchi, Sara
AU - Tovar-Lopez, Francisco J
AU - Nahavandi, Saeid
AU - Wlodkowic, Donald
AU - Mitchell, Arnan
AU - Kalantar-Zadeh, Kourosh
PY - 2012
Y1 - 2012
N2 - Abstract Dielectrophoresis, the induced motion of dielectric particles in non-uniform electric fields, enables the separation of suspended bio-particles based on their dimensions or dielectric properties. This work presents a microfluidic system, which utilises a combination of dielectrophoretic (DEP) and hydrodynamic drag forces to separate Lactobacillus bacteria from a background of yeasts. The performance of the system is demonstrated at two operating frequencies of 10 MHz and 100 kHz. At 10 MHz, we are able to trap the yeasts and bacteria at different locations of the microelectrodes as they experience different magnitudes of DEP force. Alternatively, at 100 kHz we are able to trap the bacteria along the microelectrodes, while repelling the yeasts from the microelectrodes and washing them away by the drag force. These separation mechanisms might be applicable to automated
lab-on-a-chip systems for the rapid and label-free separation of target bio-particles.
AB - Abstract Dielectrophoresis, the induced motion of dielectric particles in non-uniform electric fields, enables the separation of suspended bio-particles based on their dimensions or dielectric properties. This work presents a microfluidic system, which utilises a combination of dielectrophoretic (DEP) and hydrodynamic drag forces to separate Lactobacillus bacteria from a background of yeasts. The performance of the system is demonstrated at two operating frequencies of 10 MHz and 100 kHz. At 10 MHz, we are able to trap the yeasts and bacteria at different locations of the microelectrodes as they experience different magnitudes of DEP force. Alternatively, at 100 kHz we are able to trap the bacteria along the microelectrodes, while repelling the yeasts from the microelectrodes and washing them away by the drag force. These separation mechanisms might be applicable to automated
lab-on-a-chip systems for the rapid and label-free separation of target bio-particles.
UR - http://link.springer.com/content/pdf/10.1007%2Fs10404-011-0900-8.pdf
U2 - 10.1007/s10404-011-0900-8
DO - 10.1007/s10404-011-0900-8
M3 - Article
SN - 1613-4982
VL - 12
SP - 597
EP - 606
JO - Microfluidics and Nanofluidics
JF - Microfluidics and Nanofluidics
IS - 1-4
ER -