On-chip biochemistry: Droplet generation and control

A. Neild, D. J. Collins, M. Sesen, T. Alan

Research output: Chapter in Book/Report/Conference proceedingConference PaperResearch

Abstract

Digital microfluidics, the use of small sample droplets in oil filled channels, offers exciting possibilities in biochemistry. With the ability to form nano or picolitre droplets, there is potential for massive miniaturisation of high throughput screening (HTS) processes, where the full functionality of microtitre plate technology is replicated on a single chip for improved efficiency and substantially reduced costs. To implement such a system, the ability to create reagent mixtures on-chip in multiple permutations is required. For this goal, conventional droplet formation approaches are not well suited, where streams of hundereds of single-reagent droplets are created continuously, whereas applications such as HTS require the time-controlled creation of individual droplets. In this work, we examine the use of surface acoustic wave (SAW) excitation as a method to first generate single droplets on-demand, and then control their subsequent behaviour such that a platform for on-chip HTS can be developed. In addition, we examine methods of particle handling suitable for their introduction, in small numbers, into droplets, with the future potential for single cell encapsulation.

Original languageEnglish
Title of host publicationProceedings of the 19th Australasian Fluid Mechanics Conference, AFMC 2014
Subtitle of host publicationMelbourne, Australia; 8-11 December 2014
PublisherAustralasian Fluid Mechanics Society
Number of pages3
ISBN (Electronic)9780646596952
Publication statusPublished - 2014
EventAustralasian Fluid Mechanics Conference 2014 - Melbourne, Australia
Duration: 8 Dec 201411 Dec 2014
Conference number: 19th

Conference

ConferenceAustralasian Fluid Mechanics Conference 2014
Abbreviated titleAFMC 2014
CountryAustralia
CityMelbourne
Period8/12/1411/12/14

Cite this

Neild, A., Collins, D. J., Sesen, M., & Alan, T. (2014). On-chip biochemistry: Droplet generation and control. In Proceedings of the 19th Australasian Fluid Mechanics Conference, AFMC 2014: Melbourne, Australia; 8-11 December 2014 [483] Australasian Fluid Mechanics Society.
Neild, A. ; Collins, D. J. ; Sesen, M. ; Alan, T. / On-chip biochemistry : Droplet generation and control. Proceedings of the 19th Australasian Fluid Mechanics Conference, AFMC 2014: Melbourne, Australia; 8-11 December 2014. Australasian Fluid Mechanics Society, 2014.
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Neild, A, Collins, DJ, Sesen, M & Alan, T 2014, On-chip biochemistry: Droplet generation and control. in Proceedings of the 19th Australasian Fluid Mechanics Conference, AFMC 2014: Melbourne, Australia; 8-11 December 2014., 483, Australasian Fluid Mechanics Society, Australasian Fluid Mechanics Conference 2014, Melbourne, Australia, 8/12/14.

On-chip biochemistry : Droplet generation and control. / Neild, A.; Collins, D. J.; Sesen, M.; Alan, T.

Proceedings of the 19th Australasian Fluid Mechanics Conference, AFMC 2014: Melbourne, Australia; 8-11 December 2014. Australasian Fluid Mechanics Society, 2014. 483.

Research output: Chapter in Book/Report/Conference proceedingConference PaperResearch

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AU - Sesen, M.

AU - Alan, T.

PY - 2014

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N2 - Digital microfluidics, the use of small sample droplets in oil filled channels, offers exciting possibilities in biochemistry. With the ability to form nano or picolitre droplets, there is potential for massive miniaturisation of high throughput screening (HTS) processes, where the full functionality of microtitre plate technology is replicated on a single chip for improved efficiency and substantially reduced costs. To implement such a system, the ability to create reagent mixtures on-chip in multiple permutations is required. For this goal, conventional droplet formation approaches are not well suited, where streams of hundereds of single-reagent droplets are created continuously, whereas applications such as HTS require the time-controlled creation of individual droplets. In this work, we examine the use of surface acoustic wave (SAW) excitation as a method to first generate single droplets on-demand, and then control their subsequent behaviour such that a platform for on-chip HTS can be developed. In addition, we examine methods of particle handling suitable for their introduction, in small numbers, into droplets, with the future potential for single cell encapsulation.

AB - Digital microfluidics, the use of small sample droplets in oil filled channels, offers exciting possibilities in biochemistry. With the ability to form nano or picolitre droplets, there is potential for massive miniaturisation of high throughput screening (HTS) processes, where the full functionality of microtitre plate technology is replicated on a single chip for improved efficiency and substantially reduced costs. To implement such a system, the ability to create reagent mixtures on-chip in multiple permutations is required. For this goal, conventional droplet formation approaches are not well suited, where streams of hundereds of single-reagent droplets are created continuously, whereas applications such as HTS require the time-controlled creation of individual droplets. In this work, we examine the use of surface acoustic wave (SAW) excitation as a method to first generate single droplets on-demand, and then control their subsequent behaviour such that a platform for on-chip HTS can be developed. In addition, we examine methods of particle handling suitable for their introduction, in small numbers, into droplets, with the future potential for single cell encapsulation.

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Neild A, Collins DJ, Sesen M, Alan T. On-chip biochemistry: Droplet generation and control. In Proceedings of the 19th Australasian Fluid Mechanics Conference, AFMC 2014: Melbourne, Australia; 8-11 December 2014. Australasian Fluid Mechanics Society. 2014. 483