Oil transfer converts phosphatidylcholine vesicles into nonlamellar lyotropic liquid crystalline particles

Isabelle Martiel, Stephan Handschin, Wye-Khay Fong, Laurent Sagalowicz, Raffaele Mezzenga

Research output: Contribution to journalArticleResearchpeer-review

12 Citations (Scopus)


There is a need for the development of low-energy dispersion methods tailored to the formation of phospholipid-based nonlamellar lyotropic liquid crystalline (LLC) particles for delivery system applications. Here, facile formation of nonlamellar LLC particles was obtained by simple mixing of a phosphatidylcholine (PC) liposome solution and an oil-in-water emulsion, with limonene or isooctane as an oil. The internal structure of the particles was controlled by the PC-to-oil ratio, consistently with the sequence observed in bulk phase. For the first time, reverse micellar cubosomes with Fmm inner structure were produced. The size, morphology, and inner structure of the particles were characterized by small-angle X-ray scattering (SAXS), dynamic light scattering (DLS), and freeze-fracture cryo scanning electron microscopy (cryo-SEM). These findings pave the way to new strategies in low-energy formulation of LLC delivery systems.
Original languageEnglish
Pages (from-to)96-104
Number of pages9
Issue number1
Publication statusPublished - 2015

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